First report about saxitoxins in freshwater fish Hoplias malabaricus through trophic exposure

被引：50
作者：
da Silva, Cesar A. [2 ]
Oba, Eliane T. [1 ]
Ramsdorf, Wanessa A. [3 ]
Magalhaes, Valeria E. [5 ]
Cestari, Marta M. [3 ]
Oliveira Ribeiro, Ciro Alberto [4 ]
Silva de Assis, Helena C. [1 ]
机构：
[1] Univ Fed Parana, Dept Farmacol, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[2] Univ Fed Parana, Programa Posgrad Ecol & Conservaccao, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[3] Univ Fed Parana, Dept Genet, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[4] Univ Fed Parana, Dept Biol Celular, Setor Ciencias Biol, BR-81531970 Curitiba, Parana, Brazil
[5] Univ Fed Rio de Janeiro, Ilha Fundao, Inst Biofis Carlos Chagas Filho, BR-21941902 Rio De Janeiro, Brazil
来源：
TOXICON | 2011年 / 57卷 / 01期
关键词：
Eutrophization; Traira; Cyanotoxin; Genotocity; Paralytic Shellfish Poison; PARALYTIC SHELLFISH TOXINS; POISONING TOXINS; CELL SHRINKAGE; ACCUMULATION; GLUTATHIONE; INHIBITION; TISSUE; SALMON; TETRODOTOXIN; QUANTITATION;
D O I：
10.1016/j.toxicon.2010.10.015
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Cyanobacterial waterblooms, such as the saxitoxin (STX) producer Cylindrospermopsis raciborskii, have been a worldwide concern in environmental health. However, the bioaccumulation of this neurotoxin in the trophic chain is not completely known. The aim of the present work was to evaluate STX bioaccumulation through chemical analyses and the toxic and trophic effects using biomarkers in the tropical freshwater fish Hoplias malabaricus. They were fed once every five days with Astyanax sp. before being subjected to intraperitoneal inoculation with SIX extract (0.08 mu g/100 g) obtained by lysis of toxic C raciborskii strain (T3). After 20 days the brain was collected for acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione (GSH), lipoperoxidation (LPO), protein carbonylation (PCO), and comet assay analysis. The muscle was collected for STX chemical analysis. The activities of SOD and concentrations of PCO and LPO increased. The CAT, GST, and GPx activities decreased. Genotoxicity was observed in the experimental group. STX was not detected in muscle samples. Thus, an oxidative stress was observed in the brain, leading to the damage of lipids, proteins, and DNA. The mechanism of action of the neurotoxin in this subchronic exposure suggests an apoptotic cellular process. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：141 / 147
页数：7
相关论文
共 78 条
[1]  
AEBI H, 1984, METHOD ENZYMOL, V105, P121
[2]   Morphological and neurotoxicological findings in tropical freshwater fish (Astyanax sp.) after waterborne and acute exposure to water soluble fraction (WSF) of crude oil [J].
Akaishi, FM ;
de Assis, HCS ;
Jakobi, SCG ;
Eiras-Stofella, DR ;
St-Jean, SD ;
Courtenay, SC ;
Lima, EF ;
Wagener, ALR ;
Scofield, AL ;
Ribeiro, CAO .
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 2004, 46 (02) :244-253
[3]  
[Anonymous], ENV TOXICOL
[4]   Adaptive response of antioxidant enzymes to catalase inhibition by aminotriazole in goldfish liver and kidney [J].
Bagnyukova, TV ;
Storey, KB ;
Lushchak, VI .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 2005, 142 (03) :335-341
[5]   Effects of algal-produced neurotoxins on metabolic activity in telencephalon, optic tectum and cerebellum of Atlantic salmon (Salmo salar) [J].
Bakke, Marit Jorgensen ;
Horsberg, Tor Einar .
AQUATIC TOXICOLOGY, 2007, 85 (02) :96-103
[6]   Cell shrinkage and monovalent cation fluxes: Role in apoptosis [J].
Bortner, Carl D. ;
Cidlowski, John A. .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2007, 462 (02) :176-188
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   BETA-AMYLOID PEPTIDE FREE-RADICAL FRAGMENTS INITIATE SYNAPTOSOMAL LIPOPEROXIDATION IN A SEQUENCE-SPECIFIC FASHION - IMPLICATIONS TO ALZHEIMERS-DISEASE [J].
BUTTERFIELD, DA ;
HENSLEY, K ;
HARRIS, M ;
MATTSON, M ;
CARNEY, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1994, 200 (02) :710-715
[9]   The inner mitochondrial membrane has aquaporin-8 water channels and is highly permeable to water [J].
Calamita, G ;
Ferri, D ;
Gena, P ;
Liquori, GE ;
Cavalier, A ;
Thomas, D ;
Svelto, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (17) :17149-17153
[10]   The toxigenic marine dinoflagellate Alexandrium tamarense as the probable cause of mortality of caged salmon in Nova Scotia [J].
Cembella, A. D. ;
Quilliam, M. A. ;
Lewis, N. I. ;
Bauder, A. G. ;
Dell'Aversano, C. ;
Thomas, K. ;
Jellett, J. ;
Cusack, R. R. .
HARMFUL ALGAE, 2002, 1 (03) :313-325
12345678