Human insulin gene expressing with Bombyx mori multiple nucleopolyhedrovirus (BmMNPV) expression system

被引：0

作者：

Yue, Wan-fu ^{[1
,2
]}

Zhou, Fang ^{[1
]}

Hu, Jia-biao ^{[1
]}

Park, Enoch Y. ^{[2
]}

Hull, Joe ^{[3
]}

Miao, Yun-gen ^{[1
,3
]}

机构：

[1] Zhejiang Univ, Key Lab Anim Epidem Etiol & Immunol Prevent, Minist Agr, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China

[2] Shizuoka Univ, Biotechnol Lab, Dept Appl Biol Chem, Fac Agr,Suruga Ku, Shizuoka 4228529, Japan

[3] Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2011年 / 27卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Human insulin; Multiple nucleopolyhedrovirus; Silkworm Bombyx mori; Expression; NUCLEAR POLYHEDROSIS-VIRUS; TO-BAC/BMNPV BACULOVIRUS; MANGANESE SUPEROXIDE-DISMUTASE; INSECT CELL SYSTEM; SILKWORM LARVAE; PROTEIN;

D O I：

10.1007/s11274-010-0470-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Using human genomic DNA as a template, the human insulin gene was cloned and used to construct various reBmMNPVbacmids. Cysteine protease gene deletion (CPD-BmMNPV bacmid) and cysteine protease- and chitinase-deficient (CPPD- BmMNPV bacmid) baculoviruses were used to express both native and FLAG-tagged human insulin. Silkworm larvae were infected with the above recombinant bacmid DNAs, and the expressed insulin was purified and identified from infected silkworm haemolymph. The highest expression was shown with the CPPD- BmMNPV bacmid, which was about two times that of the wild type of reBmMNPVbacmid, reaching 15.827 ng/ml haemolymph.

引用

页码：393 / 399

页数：7

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