Hypericin can cross barriers in the chicken's chorioallantoic membrane model when delivered in low-density lipoproteins

被引：14
作者：
Blascakova, Ludmila [1 ,2 ]
Horvath, Denis [2 ]
Belej, Dominik [1 ]
Wagnieres, Georges [3 ]
Miskovsky, Pavol [2 ,4 ]
Jancura, Daniel [1 ,2 ]
Huntosova, Veronika [2 ]
机构：
[1] PJ Safarik Univ Kosice, Fac Sci, Dept Biophys, Jesenna 5, Kosice 04154, Slovakia
[2] PJ Safarik Univ Kosice, Ctr Interdisciplinary Biosci, Technol & Innovat Pk,Jesenna 5, Kosice 04154, Slovakia
[3] Swiss Fed Inst Technol Lausanne EPFL, Inst Phys, Lab Funct & Metab Imaging, Stn 6,Batiment Chim, CH-1015 Lausanne, Switzerland
[4] SAFTRA Photon Ltd, Jesenna 5, Kosice 04154, Slovakia
来源：
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | 2018年 / 23卷
基金：
瑞士国家科学基金会;
关键词：
Lipoproteins; Biodistribution; In ovo; Hypericin; Bootstrapping and bagging; PHOTODYNAMIC THERAPY; PHOTOSENSITIZER HYPERICIN; CANCER; CELLS; RECEPTOR; LUMINESCENCE; AGGREGATION; MECHANISMS; PRINCIPLES; DYNAMICS;
D O I：
10.1016/j.pdpdt.2018.07.009
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: Low-density lipoproteins (LDL) were used as a natural drug delivery system for the transport of hypericin (Hyp) in the bloodstream of the chicken's chorioallantoic membrane model (CAM). Hyp was chosen as a model for hydrophobic drug used in photo-diagnosis and photo-treatments (PDT). The extravasation of the Hyp:LDL complexes for different concentration ratios and the redistribution of Hyp between different serum components were investigated with an innovative statistical treatment. Methods: Hyp biodistribution was monitored in CAM by intravital fluorescence microscopy. The innovative statistical treatment of experimental data presented here enabled us to obtain highly detailed information from the weak Hyp fluorescence distribution in CAM blood vessels. Hyp redistribution between the serum components was studied by fluorescence spectroscopy in lipids/protein composed solutions. Results: Extravasation of Hyp was dependent on Hyp:LDL concentration ratio. While Hyp:LDL = 50:1 resulted in a significant Hyp extravasation, the Hyp extravasation from Hyp:LDL = 100:1 was weak. The redistribution of Hyp was found to be faster for lipidic particles than for proteins. Conclusion: We have demonstrated that lipids composition has a significant control over Hyp delivery in CAM.
引用
收藏
页码：306 / 313
页数：8
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