Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant

被引:30
作者
Dey, Biswajit [1 ]
Bhunia, Sanjoy K. [1 ]
Maity, Kankan K. [1 ]
Patra, Sukesh [1 ]
Mandal, Soumitra [1 ]
Maiti, Swatilekha [2 ]
Maiti, Tapas K. [2 ]
Sikdar, Samir R. [3 ]
Islam, Syed S. [1 ]
机构
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India
[3] Bose Inst, Plant Mol & Cellular Genet Sect, Kolkata 700054, W Bengal, India
关键词
Pleurotus florida blue variant; Mushroom polysaccharide; Structure; NMR spectroscopy; Immunoactivation; SAJOR-CAJU; AQUEOUS EXTRACT; STRUCTURAL-CHARACTERIZATION; ANTITUMOR POLYSACCHARIDES; CHINESE MUSHROOM; FRUITING BODY; OSTREATUS; GLUCAN; (1->3)-BETA-D-GLUCAN; CONFORMATION;
D O I
10.1016/j.carres.2010.09.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom Pleurotus florida blue variant was found to consist of D-glucose and D-galactose in a molar ratio of nearly 5 1 On the basis of sugar analysis methylation analysis periodate oxidation Smith degradation and NMR studies (H-1 C-13 DEPT-135 DQF-COSY TOCSY NOESY ROESY HMQC and HMBC) the structure of the repeating unit of the polysaccharide was established as [GRAPHICS] The molecule activated macrophages splenocytes and thymocytes (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码:2736 / 2741
页数:6
相关论文
共 33 条
[11]  
KHANNA P, 1981, MUSHROOM SCI, V11, P561
[12]   Antiproliferative and immunostimulatory protein fraction from edible mushrooms [J].
Maiti, Swatilekha ;
Bhutia, Sujit K. ;
Mallick, Sanjaya K. ;
Kumar, Alok ;
Khadgi, Niyad ;
Maiti, Tapas K. .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2008, 26 (02) :187-191
[13]   Structural studies of a heteropolysaccharide (PS-I) isolated from hot water extract of fruits of Psidium guajava']java (Guava) [J].
Mandal, Soumitra ;
Sarkar, Ramsankar ;
Patra, Pradip ;
Nandan, Chanchal K. ;
Das, Debsankar ;
Bhanja, Sunil K. ;
Islam, Syed S. .
CARBOHYDRATE RESEARCH, 2009, 344 (11) :1365-1370
[14]   Isolation and structural elucidation of a water-soluble polysaccharide (PS-I) of a wild edible mushroom, Termitomyces striatus [J].
Mondal, S ;
Chakraborty, I ;
Rout, D ;
Islam, SS .
CARBOHYDRATE RESEARCH, 2006, 341 (07) :878-886
[15]   Conformation dependency of nitric oxide synthesis of murine peritoneal macrophages by beta-glucans in vitro [J].
Ohno, N ;
Hashimoto, T ;
Adachi, Y ;
Yadomae, T .
IMMUNOLOGY LETTERS, 1996, 52 (01) :1-7
[16]  
OHNO N, 1993, BIOL PHARM BULL, V16, P414
[17]   Glucans from the alkaline extract of an edible mushroom, Pleurotus florida, cv Assam Florida: isolation, purification, and characterization [J].
Ojha, Arnab K. ;
Chandra, Krishnendu ;
Ghosh, Kaushik ;
Islam, Syed S. .
CARBOHYDRATE RESEARCH, 2010, 345 (15) :2157-2163
[18]   Structural characterization of an immunoenhancing heteropolysaccharide isolated from hot water extract of the fresh leaves of Catharanthus rosea [J].
Patra, Sukesh ;
Maity, Kankan K. ;
Bhunia, Sanjoy K. ;
Dey, Biswajit ;
Das, Debsankar ;
Mondal, Soumitra ;
Bhunia, Bibhas ;
Maiti, Tapas K. ;
Islam, Syed. S. .
CARBOHYDRATE POLYMERS, 2010, 81 (03) :584-591
[19]   Structural investigation of a polysaccharide (Fr. II) isolated from the aqueous extract of an edible mushroom, Pleurotus sajor-caju [J].
Pramanik, M ;
Mondal, S ;
Chakraborty, I ;
Rout, D ;
Islam, SS .
CARBOHYDRATE RESEARCH, 2005, 340 (04) :629-636
[20]   Structural analysis of a water-soluble glucan (Fr.I) of an edible mushroom, Pleurotus sajor-caju [J].
Pramanik, Malay ;
Chakraborty, Indranil ;
Mondal, Soumitra ;
Islam, Syed S. .
CARBOHYDRATE RESEARCH, 2007, 342 (17) :2670-2675