Chemical analysis of an immunoenhancing water-soluble polysaccharide of an edible mushroom, Pleurotus florida blue variant

被引:30
作者
Dey, Biswajit [1 ]
Bhunia, Sanjoy K. [1 ]
Maity, Kankan K. [1 ]
Patra, Sukesh [1 ]
Mandal, Soumitra [1 ]
Maiti, Swatilekha [2 ]
Maiti, Tapas K. [2 ]
Sikdar, Samir R. [3 ]
Islam, Syed S. [1 ]
机构
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India
[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India
[3] Bose Inst, Plant Mol & Cellular Genet Sect, Kolkata 700054, W Bengal, India
关键词
Pleurotus florida blue variant; Mushroom polysaccharide; Structure; NMR spectroscopy; Immunoactivation; SAJOR-CAJU; AQUEOUS EXTRACT; STRUCTURAL-CHARACTERIZATION; ANTITUMOR POLYSACCHARIDES; CHINESE MUSHROOM; FRUITING BODY; OSTREATUS; GLUCAN; (1->3)-BETA-D-GLUCAN; CONFORMATION;
D O I
10.1016/j.carres.2010.09.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An immunoenhancing polysaccharide isolated from the aqueous extract of the fruit bodies of the mushroom Pleurotus florida blue variant was found to consist of D-glucose and D-galactose in a molar ratio of nearly 5 1 On the basis of sugar analysis methylation analysis periodate oxidation Smith degradation and NMR studies (H-1 C-13 DEPT-135 DQF-COSY TOCSY NOESY ROESY HMQC and HMBC) the structure of the repeating unit of the polysaccharide was established as [GRAPHICS] The molecule activated macrophages splenocytes and thymocytes (C) 2010 Elsevier Ltd All rights reserved
引用
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页码:2736 / 2741
页数:6
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