Effects of (+)catechin and (-)epicatechin on heterocyclic amines-induced oxidative DNA damage

被引:33
作者
Haza, Ana Isabel [1 ]
Morales, Paloma [1 ]
机构
[1] Univ Complutense Madrid, Fac Vet, Dept Nutr Bromatol & Tecnol Alimentos, E-28040 Madrid, Spain
关键词
(+)-catechin; (-)-epicatechin; comet assay; heterocyclic amines; oxidative DNA damage; AROMATIC-AMINES; DIETARY FLAVONOIDS; ADDUCT FORMATION; STRAND BREAKS; POLYPHENOLS; METABOLISM; INHIBITION; INDUCTION; FOOD; CYTOCHROME-P450;
D O I
10.1002/jat.1559
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
The aim of the present study was to evaluate the protective effect of (+)-catechin and (-)-epicatechin against 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (8-MelQx), 2-amino-3,4,8-trimethylimidazo[4,5f]-quinoxaline (4,8-diMelQx) and 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine (PhIP)-induced DNA damage in human hepatoma cells (HepG2). DNA damage (strand breaks and oxidized purines/pyrimidines) was evaluated by the alkaline single-cell gel electrophoresis or comet assay. Increasing concentrations of 8-MelQx, 4,8-diMelQx and PhIP induced a significant increase in DNA strand breaks and oxidized purines and pyrimidines in a dose-dependent manner. Among those, PhIP (300 ion) exerted the highest genotoxicity. (+)-Catechin exerted protection against oxidized purines induced by 8-MelQx, 4,8-diMelQx and PhIP. Oxidized pyrimidines and DNA strand breaks induced by PhIP were also prevented by (+)-catechin. Otherwise, (-)-epicatechin protected against the oxidized pyrimidines induced by PhIP and the oxidized purines induced by 8-MelQx and 4,8-diMelQx. One feasible mechanism by which (+)-catechin and (-)-epicatechin exert their protective effect towards heterocyclic amines-induced oxidative DNA damage may be by modulation of phase land II enzyme activities. The ethoxyresorufin O-deethylation (CYP1A1) activity was moderately inhibited by (+)-catechin, while little effect was observed by (-)-epicatechin. However, (+)-catechin showed the greatest increase in UDP-glucuronyltransferase activity. In conclusion, our results clearly indicate that (+)-catechin was more efficient than (-)-epicatechin in preventing DNA damage (strand breaks and oxidized purines/pyrimidines) induced by PhIP than that induced by 8-MelQx and 4,8-diMelQx. Copyright (C) 2010 John Wiley & Sons, Ltd.
引用
收藏
页码:53 / 62
页数:10
相关论文
共 50 条
  • [41] The role of oxidative DNA damage in radiation induced bystander effect
    Havaki, Sophia
    Kotsinas, Athanassios
    Chronopoulos, Efstathios
    Kletsas, Dimitris
    Georgakilas, Alexandros
    Gorgoulis, Vassilis G.
    CANCER LETTERS, 2015, 356 (01) : 43 - 51
  • [42] Mechanisms and measurements of nanomaterial-induced oxidative damage to DNA
    Petersen, Elijah J.
    Nelson, Bryant C.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 398 (02) : 613 - 650
  • [43] Tools and Approaches for the Assessment of Nanomaterial Induced Oxidative DNA Damage
    Petersen, E. J.
    Marquis, B. J.
    Jaruga, P.
    Dizdaroglu, M.
    Nelson, B. C.
    NANOTECHNOLOGY 2012, VOL 3: BIO SENSORS, INSTRUMENTS, MEDICAL, ENVIRONMENT AND ENERGY, 2012, : 307 - 310
  • [44] Determination of DNA damage induced by oxidative stress in hyperlipidemic patients
    Harangi, M
    Remenyik, É
    Seres, I
    Varga, Z
    Katona, E
    Paragh, G
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2002, 513 (1-2) : 17 - 25
  • [45] Oxidative DNA damage and glioma cell death induced by tetrahydropapaveroline
    Soh, Y
    Shin, MH
    Lee, JS
    Jang, JH
    Kim, OH
    Kang, H
    Surh, YJ
    MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2003, 544 (2-3) : 129 - 142
  • [46] Paraquat induced oxidative stress, DNA damage, and cytotoxicity in lymphocytes
    Alizadeh, Soheila
    Anani-sarab, Gholamreza
    Amiri, Hoda
    Hashemi, Majid
    HELIYON, 2022, 8 (07)
  • [47] Radio-modulatory effects of Green Tea Catechin EGCG on pBR322 plasmid DNA and murine splenocytes against gamma-radiation induced damage
    Richi, Benila
    Kale, Raosaheb K.
    Tiku, Ashu B.
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2012, 747 (01) : 62 - 70
  • [48] Hypoxia-induced oxidative DNA damage links with higher level biological effects including specific growth rate in common carp, Cyprinus carpio L.
    Mustafa, Sanaa A.
    Al-Subiai, Sherain N.
    Davies, Simon J.
    Jha, Awadhesh N.
    ECOTOXICOLOGY, 2011, 20 (06) : 1455 - 1466
  • [49] Effects of basal level of antioxidants on oxidative DNA damage in humans
    Marek Foksinski
    Daniel Gackowski
    Rafal Rozalski
    Agnieszka Siomek
    Jolanta Guz
    Anna Szpila
    Tomasz Dziaman
    Ryszard Olinski
    European Journal of Nutrition, 2007, 46
  • [50] Effects of basal level of antioxidants on oxidative DNA damage in humans
    Foksinski, Marek
    Gackowski, Daniel
    Rozalski, Rafal
    Siomek, Agnieszka
    Guz, Jolanta
    Szpila, Anna
    Dziaman, Tomasz
    Olinski, Ryszard
    EUROPEAN JOURNAL OF NUTRITION, 2007, 46 (03) : 174 - 180