Feasibility of enzyme biosensors based on gold nanowires

Gold nanowires were prepared by electroless deposition within the pores of polycarbonate particle track-etched membranes (PTM). Glucose oxidase was deposited onto the nanowires using self-assembling monolayer as anchor layers for the enzyme molecules. AFM characterization was performed before and after the immobilization of the enzyme. Finally cyclic voltammetry was performed for different enzymes to test the applicability of gold nanowires as biosensors. The AFM measurements proved the immobilization of the enzyme while the electrochemical characterization showed that the presence of nanowired substrates greatly enhances the electrical response of the biosensor. (c) 2006 Elsevier B.V. All rights reserved.