Combinatorial in vitro RNAi of two neuropeptide genes and a pharyngeal gland gene on Meloidogyne incognita

被引：11

作者：

Banakar, Prakash ^{[1
]}

Sharma, Amita ^{[1
]}

Lilley, Catherine J. ^{[2
]}

Gantasala, Nagavara Prasad ^{[1
]}

Kumar, Mukesh ^{[1
]}

Rao, Uma ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Nematol, New Delhi 110012, India

[2] Univ Leeds, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England

来源：

NEMATOLOGY | 2015年 / 17卷

基金：

英国工程与自然科学研究理事会;

关键词：

double-stranded RNA; FMRFamide-like peptides; neuromotor function; qRT-PCR; root-knot nematode; sensory; ROOT-KNOT NEMATODES; NERVOUS-SYSTEM; CAENORHABDITIS-ELEGANS; DISRUPTION; NEURONS; FAMILY;

D O I：

10.1163/15685411-00002859

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Root-knot nematodes are the most economically important group of plant-parasitic nematodes. In the present study, functional validation using in vitro RNAi was carried out on Meloidogyne incognita with two FMRFamide-like peptide genes, flp-14 and flp-18, and a subventral pharyngeal gland specific gene, 16D10. It was found that RNAi silencing of each gene reduced the attraction of M. incognita at different time intervals both in combination and individually. Silencing of the genes reduced nematode infection by 23-30% and development as indicated by a reduction in the number of females by 26-62%. Reproduction was decreased by 27-73% and fecundity was decreased by 19-51%. In situ hybridisation revealed the expression of flp-18 in cells associated with the ventral and retro vesicular ganglia of the central nervous system. qRT-PCR supported the correlation between phenotypic effects of silencing with that of transcript quantification.

引用

页码：155 / 167

页数：13

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