Cell and antibody mediated immunity induced by vaccination with novel Candida dubliniensis mannan immunogenic conjugate

被引：17
作者：
Paulovicova, Ema
Machova, Eva
Tuhnska, Jana
Bystricky, Slavomir
机构：
[1] Slovak Acad Sci, Inst Chem, Bratislava 84538, Slovakia
[2] Res Base Slovak Med Univ, Inst Prevent & Clin Med, Lab Immunotoxicol, Bratislava 83303, Slovakia
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2007年 / 7卷 / 10期
关键词：
C. dubliniensis mannan; polysaccharide-protein conjugate; cellular immunity; immunoglobulins;
D O I：
10.1016/j.intimp.2007.05.014
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Antigen-specific Immoral response, as well as the induction of cellular immunity generated by Candida dubliniensis mannan-human serum albumin (HSA) conjugate, a novel proposed immunogenic structure for subcellular vaccine, were evaluated in rabbits. Mannan-HSA conjugate-induced specific IgG and IgA increased significantly after boosters (IgG: P < 0.001 and IgA: P < 0.01). Mannan-HSA conjugate up-regulation of cell-surface expression of B-lymphocyte and granulocyte activation antigens CD25 and CD11b indicated the effective activation. Immunogenic effect of conjugate on T lymphocytes was demonstrated via inductive increase of CD4(+) T lymphocyte subset and CD4(+)/CD8(+) ratio and via induction of T-H(l) cytokines. Immunogenic effectiveness of mannan-HSA conjugate at a dose of 0.25 mg of mannan antigenic moiety overcame that of the mannan alone and of yeast whole cells, thus promising further application in Candida vaccine development. (c) 2007 Elsevier B.V. All rights reserved.
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收藏
页码：1325 / 1333
页数：9
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