Clostridium difficile PCR ribotypes 001 and 176-the common denominator of C. difficile infection epidemiology in the Czech Republic, 2014

被引：25

作者：

Krutova, M. ^{[1
,2
,3
]}

Matejkova, J. ^{[1
,2
]}

Kuijper, E. J. ^{[4
]}

Drevinek, P. ^{[1
,2
]}

Nyc, O. ^{[1
,2
]}

机构：

[1] Charles Univ Prague, Fac Med 2, Dept Med Microbiol, CR-11636 Prague 1, Czech Republic

[2] Univ Hosp Motol, Prague, Czech Republic

[3] Charles Univ Prague, Fac Med 2, Dept Paediat Neurol, DNA Lab, Prague, Czech Republic

[4] Leiden Univ, Med Ctr, Dept Med Microbiol, Leiden, Netherlands

来源：

EUROSURVEILLANCE | 2016年 / 21卷 / 29期

关键词：

EMERGENCE; POLAND; MULTICENTER; PREVALENCE;

D O I：

10.2807/1560-7917.ES.2016.21.29.30296

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

In 2014, 18 hospitals in the Czech Republic participated in a survey of the incidence of Clostridium difficile infections (CDI) in the country. The mean CDI incidence was 6.1 (standard deviation (SD): 7.2) cases per 10,000 patient bed-days and 37.8 cases (SD: 41.4) per 10,000 admissions. The mean CDI testing frequency was 39.5 tests (SD: 25.4) per 10,000 patient bed-days and 255.8 tests (SD: 164.0) per 10,000 admissions. A total of 774 C. difficile isolates were investigated, of which 225 (29%) belonged to PCR ribotype 176, and 184 isolates (24%) belonged to PCR ribotype 001. Multilocus variable-number tandem repeat analysis (MLVA) revealed 27 clonal complexes formed by 84% (190/225) of PCR ribotype 176 isolates, and 14 clonal complexes formed by 77% (141/184) of PCR ribotype 001 isolates. Clonal clusters of PCR ribotypes 176 and 001 were observed in 11 and 7 hospitals, respectively. Our data demonstrate the spread of two C. difficile PCR ribotypes within 18 hospitals in the Czech Republic, stressing the importance of standardising CDI testing protocols and implementing mandatory CDI surveillance in the country.

引用

页码：58 / 68

页数：11

共 34 条

[1] The undiagnosed cases of Clostridium difficile infection in a whole nation: where is the problem? [J].

Alcala, L. ;

Martin, A. ;

Marin, M. ;

Sanchez-Somolinos, M. ;

Catalan, P. ;

Pelaez, T. ;

Bouza, E. .

CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (07) :E204-E213

[2]

[Anonymous], 2015, European surveillance of Clostridium difficile infections. Surveillance protocol version 2.2

[3]

Arvand M, 2014, Euro Surveill, V19, DOI 10.2807/1560-7917.ES2014.19.10.20732

[4] Clostridium difficile infection in Europe: a hospital-based survey [J].

Bauer, Martijn P. ;

Notermans, Daan W. ;

van Benthem, Birgit H. B. ;

Brazier, Jon S. ;

Wilcox, Mark H. ;

Rupnik, Maja ;

Monnet, Dominique L. ;

van Dissel, Jaap T. ;

Kuijper, Ed J. .

LANCET, 2011, 377 (9759) :63-73

[5] Underdiagnosis of Clostridium difficile across Europe: the European, multicentre, prospective, biannual, point-prevalence study of Clostridium difficile infection in hospitalised patients with diarrhoea (EUCLID) [J].

Davies, Kerrie A. ;

Longshaw, Christopher M. ;

Davis, Georgina L. ;

Bouza, Emilio ;

Barbut, Frederic ;

Barna, Zsuzsanna ;

Delmee, Michel ;

Fitzpatrick, Fidelma ;

Ivanova, Kate ;

Kuijper, Ed ;

Macovei, Ioana S. ;

Mentula, Silja ;

Mastrantonio, Paola ;

von Mueller, Lutz ;

Oleastro, Monica ;

Petinaki, Efthymia ;

Pituch, Hanna ;

Noren, Torbjorn ;

Novakova, Elena ;

Nyc, Otakar ;

Rupnik, Maja ;

Schmid, Daniela ;

Wilcox, Mark H. .

LANCET INFECTIOUS DISEASES, 2014, 14 (12) :1208-1219

[6] European Society of Clinical Microbiology and Infectious Diseases: update of the treatment guidance document for Clostridium difficile infection [J].

Debast, S. B. ;

Bauer, M. P. ;

Kuijper, E. J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2014, 20 :1-26

[7] Development and Validation of an Internationally-Standardized, High-Resolution Capillary Gel-Based Electrophoresis PCR-Ribotyping Protocol for Clostridium difficile [J].

Fawley, Warren N. ;

Knetsch, C. W. ;

MacCannell, Duncan R. ;

Harmanus, Celine ;

Du, Tim ;

Mulvey, Michael R. ;

Paulick, Ashley ;

Anderson, Lydia ;

Kuijper, E. J. ;

Wilcox, Mark H. .

PLOS ONE, 2015, 10 (02)

[8] Pan-European longitudinal surveillance of antibiotic resistance among prevalent Clostridium difficile ribotypes [J].

Freeman, J. ;

Vernon, J. ;

Morris, K. ;

Nicholson, S. ;

Todhunter, S. ;

Longshaw, C. ;

Wilcox, M. H. .

CLINICAL MICROBIOLOGY AND INFECTION, 2015, 21 (03) :248.e9-248.e16

[9] Application of multiple-locus variable-number tandem-repeat analysis to determine clonal spread of toxin A-negative Clostridium difficile in a general hospital in Buenos Aires, Argentina [J].

Goorhuis, A. ;

Legaria, M. C. ;

van den Berg, R. J. ;

Harmanus, C. ;

Klaassen, C. H. W. ;

Brazier, J. S. ;

Lumelsky, G. ;

Kuijper, E. J. .

CLINICAL MICROBIOLOGY AND INFECTION, 2009, 15 (12) :1080-1086

[10] Emergence and global spread of epidemic healthcare-associated Clostridium difficile [J].

He, Miao ;

Miyajima, Fabio ;

Roberts, Paul ;

Ellison, Louise ;

Pickard, Derek J. ;

Martin, Melissa J. ;

Connor, Thomas R. ;

Harris, Simon R. ;

Fairley, Derek ;

Bamford, Kathleen B. ;

D'Arc, Stephanie ;

Brazier, Jon ;

Brown, Derek ;

Coia, John E. ;

Douce, Gill ;

Gerding, Dale ;

Kim, Hee Jung ;

Koh, Tse Hsien ;

Kato, Haru ;

Senoh, Mitsutoshi ;

Louie, Tom ;

Michell, Stephen ;

Butt, Emma ;

Peacock, Sharon J. ;

Brown, Nick M. ;

Riley, Tom ;

Songer, Glen ;

Wilcox, Mark ;

Pirmohamed, Munir ;

Kuijper, Ed ;

Hawkey, Peter ;

Wren, Brendan W. ;

Dougan, Gordon ;

Parkhill, Julian ;

Lawley, Trevor D. .

NATURE GENETICS, 2013, 45 (01) :109-U155

← 1 2 3 4 →