Fibroblast growth factor-2 stimulates adipogenic differentiation of human adipose-derived stem cells

被引：127
作者：
Kakudo, Natsuko [1 ]
Shimotsuma, Ayuko [1 ]
Kusumoto, Kenji [1 ]
机构：
[1] Kansai Med Univ, Dept Plast & Reconstruct Surg, Moriguchi, Osaka 5708506, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 359卷 / 02期
关键词：
fibroblast growth factor-2; adipose-derived stem cells; adipogenic differentiation;
D O I：
10.1016/j.bbrc.2007.05.070
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adipose-derived stem cells (ASCs) have demonstrated a capacity for differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth and factors. It is acknowledged that fibroblast growth factor-2 (FGF-2) promotes chondrogenic and inhibits osteogenic differentiation of ASCs, but thorough investigations of its effects on adipogenic differentiation are lacking. In this study, we demonstrate at the cellular and molecular levels the effect of FGF-2 on adipogenic differentiation of ASCs, as induced by an adipogenic hormonal cocktail consisting of 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, insulin, and indomethacin. FGF-2 significantly enhances the adipogenic differentiation of human ASCs. Furthermore, in cultures receiving FGF-2 before adipogenic induction, mRNA expression of peroxisome proliferator-activated receptor gamma 2 (PPAR gamma 2), a key transcription factor in adipogenesis, was upregulated. The results of FGF-2 supplementation suggest the potential applications of FGF-2 and ASCs in adipose tissue regeneration. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：239 / 244
页数：6
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