Flavin-mediated photo-oxidation for the detection of mitochondrial flavins

被引：6

作者：

Lee, Dong-Nam ^{[1
]}

Kim, Eunjin ^{[2
]}

Lee, Jae Hong ^{[3
]}

Kim, Jong Seung ^{[3
]}

Kang, Chulhun ^{[2
]}

Hong, Jong-In ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Coll Nat Sci, Seoul 08826, South Korea

[2] Kyung Hee Univ, Grad Sch Med Sci, Yongin 446701, South Korea

[3] Korea Univ, Dept Chem, Seoul 136701, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 92期

关键词：

FLUORESCENCE; FLAVOPROTEIN; OXIDATION; MICROSCOPY; COENZYMES; CATALYSIS; EFFICIENT; DISEASE; COMPLEX;

D O I：

10.1039/c6cc07483a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a new approach for the detection of mitochondrial flavins through photo-oxidation of a probe molecule. Probe 1 showed high brightness (epsilon x Phi(f) = 6.50 x 10(3) M-1 cm(-1)) at long wavelengths (maximum emission wavelength, lambda(max) = 600 nm) upon photo-oxidation, assisted by the strong electron accepting ability of the isoalloxazine moiety in flavins. Probe 1 also exhibited high selectivity for flavins over various biological oxidants, remarkable photo-stability, and mitochondrial localization.

引用

页码：13487 / 13490

页数：4

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