Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry

被引：17

作者：

Ji, Eun Sun ^{[1
]}

Hwang, Heeyoun ^{[1
]}

Park, Gun Wook ^{[1
,2
]}

Lee, Ju Yeon ^{[1
]}

Lee, Hyun Kyoung ^{[1
,2
]}

Choi, Na Young ^{[1
,2
]}

Jeong, Hoi Keun ^{[1
,2
]}

Kim, Kwang Hoe ^{[1
,2
]}

Kim, Jin Young ^{[1
]}

Lee, Seungho ^{[3
]}

Ahn, Yeong Hee ^{[4
]}

Yoo, Jong Shin ^{[1
,2
]}

机构：

[1] Korea Basic Sci Inst, Biomed Omics Grp, 162 YeonGuDanji Ro, Cheongju 28119, Chungbuk, South Korea

[2] Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Daejeon 305764, South Korea

[3] Hannam Univ, Dept Chem, Daejeon 306791, South Korea

[4] Cheongju Univ, Dept Biomed Sci, Cheongju 28503, South Korea

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2016年 / 408卷 / 27期

关键词：

N-Glycoproteins; Fucosylation; Liquid chromatography; Mass spectrometry; Human plasma; Liver-secreted glycoproteins; QUANTITATIVE-ANALYSIS; GLYCOSYLATION SITES; ALPHA-FETOPROTEIN; SERUM; CANCER; PROTEIN; LECTIN; GLYCANS; IDENTIFICATION; HAPTOGLOBIN;

D O I：

10.1007/s00216-016-9878-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fucosylation of N-glycoproteins has been implicated in various diseases, such as hepatocellular carcinoma (HCC). However, few studies have performed site-specific analysis of fucosylation in liver-secreted proteins. In this study, we characterized the fucosylation patterns of liver-secreted proteins in HCC plasma using a workflow to identify site-specific N-glycoproteins, where characteristic B- and/or Y-ion series with and without fucose in collision-induced dissociation were used in tandem mass spectrometry. In total, 71 fucosylated N-glycopeptides from 13 major liver-secreted proteins in human plasma were globally identified by LC-MS/MS. Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. Of the fucosylated N-glycopeptides, bi- and tri-antennary glycoforms were the most common ones identified in liver-secreted proteins from HCC plasma. Therefore, we suggest that this analytical method is effective for characterizing fucosylation in liver-secreted proteins.

引用

页码：7761 / 7774

页数：14

共 53 条

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