Detection of single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer using a fluorophore-tagged DNA probe

被引：10

作者：

Zhao, Zheng-yun ^{[1
]}

San, Marie ^{[1
]}

Duprey, Jean-Louis H. A. ^{[1
]}

Arrand, John R. ^{[2
]}

Vyle, Joseph S. ^{[3
]}

Tucker, James H. R. ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[2] Univ Birmingham, Sch Canc Sci, Birmingham B15 2TT, W Midlands, England

[3] Queens Univ Belfast, Sch Chem & Chem Engn, Belfast BT9 5AG, Antrim, North Ireland

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 01期

基金：

英国工程与自然科学研究理事会;

关键词：

DNA; SNP; Fluorescence; Sensing; Nucleic acids; BASE; NUCLEOSIDE;

D O I：

10.1016/j.bmcl.2011.11.053

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Single nucleotide polymorphisms within a sequence of a gene associated with prostate cancer were identified using oligodeoxynucleotide probe sequences bearing internal anthracene fluorophores proximal to the SNP site. Depending upon the nature of the synthesised target sequences, probe-target duplex formation could lead to enhanced or attenuated fluorescence emission from the anthracene, enabling detection of a proximal base-pair as either matching or mismatching. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：129 / 132

页数：4

共 14 条

[1] Single base interrogation by afluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy [J].

Cekan, Pavol ;

Sigurdsson, Snorri Th. .

CHEMICAL COMMUNICATIONS, 2008, (29) :3393-3395

[2]

Chanock S.J., 2008, NAT GENET, V40, P310

[3] Intrinsically Fluorescent Base-Discriminating Nucleoside Analogs [J].

Dodd, D. W. ;

Hudson, R. H. E. .

MINI-REVIEWS IN ORGANIC CHEMISTRY, 2009, 6 (04) :378-391

[4] Detection of DNA base variation and cytosine methylation at a single nucleotide site using a highly sensitive fluorescent probe [J].

Duprey, Jean-Louis H. A. ;

Zhao, Zheng-yun ;

Bassani, Dario M. ;

Manchester, Jack ;

Vyle, Joseph S. ;

Tucker, James H. R. .

CHEMICAL COMMUNICATIONS, 2011, 47 (23) :6629-6631

[5] Anthracene-modified oligonucleotides as fluorescent DNA mismatch sensors: discrimination between various base-pair mismatches [J].

Duprey, Jean-Louis H. A. ;

Bassani, Dario M. ;

Hyde, Eva I. ;

Ludwig, Christian ;

Rodger, Alison ;

Vyle, Joseph S. ;

Wilkie, John ;

Zhao, Zheng-Yun ;

Tucker, James H. R. .

SUPRAMOLECULAR CHEMISTRY, 2011, 23 (3-4) :273-277

[6] Using high-throughput SNP technologies to study cancer [J].

Engle, LJ ;

Simpson, CL ;

Landers, JE .

ONCOGENE, 2006, 25 (11) :1594-1601

[7] Alzheimer's disease genetics: current knowledge and future challenges [J].

Hollingworth, Paul ;

Harold, Denise ;

Jones, Lesley ;

Owen, Michael J. ;

Williams, Julie .

INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, 2011, 26 (08) :793-802

[8] Single nucleotide polymorphism libraries: why and how are we building them? [J].

Kwok, PY ;

Gu, ZJ .

MOLECULAR MEDICINE TODAY, 1999, 5 (12) :538-543

[9] BASE-SEQUENCE DEPENDENCE OF EMISSION LIFETIMES FOR DNA OLIGOMERS AND DUPLEXES COVALENTLY LABELED WITH PYRENE - RELATIVE ELECTRON-TRANSFER QUENCHING EFFICIENCIES OF A-NUCLEOSIDE, G-NUCLEOSIDE, C-NUCLEOSIDE, AND T-NUCLEOSIDE TOWARD PYRENE [J].

MANOHARAN, M ;

TIVEL, KL ;

ZHAO, M ;

NAFISI, K ;

NETZEL, TL .

JOURNAL OF PHYSICAL CHEMISTRY, 1995, 99 (48) :17461-17472

[10] Detection of a single DNA base-pair mismatch using an anthracene-tagged fluorescent probe [J].

Moran, Nina ;

Bassani, Dario M. ;

Desvergne, Jean-Pierre ;

Keiper, Sonja ;

Lowden, Philip A. S. ;

Vyle, Joseph S. ;

Tucker, James H. R. .

CHEMICAL COMMUNICATIONS, 2006, (48) :5003-5005

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