Is mouse embryonic stem cell technology obsolete?

被引：13
|
作者：
Skarnes, William C. [1 ]
机构：
[1] Wellcome Trust Sanger Inst, Stem Cell Engn, Cambridge CB10 1SA, England
来源：
GENOME BIOLOGY | 2015年 / 16卷
基金：
英国惠康基金;
关键词：
GENE; MICE; GENERATION; KNOCKOUT;
D O I：
10.1186/s13059-015-0673-6
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.
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页数：3
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