Effect of acaricides on the activity of a Boophilus microplus glutathione S-transferase

被引：41

作者：

Vaz, ID

Lermen, TT

Michelon, A

Ferreira, CAS

de Freitas, DRJ

Termignoni, C

Masuda, A

机构：

[1] Univ Fed Rio Grande do Sul, Ctr Biotecnol Estado Rio Grande Sul, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Fac Vet, BR-91501970 Porto Alegre, RS, Brazil

[3] Univ Fed Rio Grande do Sul, Dept Mol Biol & Biotechnol, BR-91501970 Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Dept Bioquim, BR-91501970 Porto Alegre, RS, Brazil

[5] Univ Fed Pelotas, Ctr Biotechnol, Pelotas, RS, Brazil

来源：

VETERINARY PARASITOLOGY | 2004年 / 119卷 / 2-3期

关键词：

Boophilus microplus; glutathione S-transferase; acaricides; drug resistance;

D O I：

10.1016/j.vetpar.2003.11.004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

In the present study, we report the effect of several acaricides on the enzyme activity of a Boophilus microplus recombinant glutathione S-transferase (rGST). GST was expressed in Escherichia coli and was purified with glutathione (GSH) affinity column chromatography. The kinetic constants were determined by reacting GST with the substrates 1-chloro-2,4-dinitrobenzene (CDNB) and glutathione. We report the effect of several acaricides on the enzyme activity of rGST. Some acaricides (ethion, amitraz, chlorpyrifos, DDT, cypermethrin, diazinon, ivermectin, deltamethrin and flumethrin) inhibited rGST. Contrarily, coumaphos had an activating effect. Although the accurate mechanisms of the B. microplus resistance to acaricides remain elusive, this work helps in understanding how acaricides can interact with GST. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：237 / 245

页数：9

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