Targeted genome editing in primate embryos

被引：30

作者：

Guo, Xiangyu ^{[1
]}

Li, Xiao-Jiang ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[2] Emory Univ, Sch Med, Dept Human Genet, Atlanta, GA 30322 USA

来源：

CELL RESEARCH | 2015年 / 25卷 / 07期

关键词：

CRISPR/CAS SYSTEM; CYNOMOLGUS MONKEY; GENE; CRISPR-CAS9; GENERATION; EFFICIENCY; CELLS; MICE;

D O I：

10.1038/cr.2015.64

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mosaic mutations and off-target effects caused by CRISPR/Cas9 have led to concerns about the efficiency and specificity of this new technique in non-human primates and other large animals. Here we discuss recent findings from primate embryos, with a focus on the technical issues CRISPR/Cas9 faces before producing non-human primate models of human diseases.

引用

页码：767 / 768

页数：2

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