Quantitative phase microscopy for cellular dynamics based on transport of intensity equation

被引：68

作者：

Li, Ying ^{[1
]}

Di, Jianglei ^{[1
]}

Ma, Chaojie ^{[1
]}

Zhang, Jiwei ^{[1
]}

Zhong, Jinzhan ^{[1
]}

Wang, Kaiqiang ^{[1
]}

Xi, Teli ^{[1
]}

Zhao, Jianlin ^{[1
]}

机构：

[1] Northwestern Polytech Univ, Shaanxi Key Lab Opt Informat Technol, MOE Key Lab Mat Phys & Chem Extraordinary Condit, Sch Sci, Xian 710072, Shaanxi, Peoples R China

来源：

OPTICS EXPRESS | 2018年 / 26卷 / 01期

基金：

中国国家自然科学基金;

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; RETRIEVAL; INTERFEROMETRY; ILLUMINATION; CONTRAST; LIGHT;

D O I：

10.1364/OE.26.000586

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a simple method for quantitative phase imaging of tiny transparent objects such as living cells based on the transport of intensity equation. The experiments are performed using an inverted bright field microscope upgraded with a flipping imaging module, which enables to simultaneously create two laterally separated images with unequal defocus distances. This add-on module does not include any lenses or gratings and is cost-effective and easy-to-alignment. The validity of this method is confirmed by the measurement of microlens array and human osteoblastic cells in culture, indicating its potential in the applications of dynamically measuring living cells and other transparent specimens in a quantitative, non-invasive and label-free manner. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：586 / 593

页数：8

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