Platelet-derived growth factor BB is reduced in endometrial endothelial cells of women with abnormal uterine bleeding-endometrial disorder

被引:1
作者
Shivhare, Sourima Biswas [1 ]
Lu, Qinsheng [2 ]
Sun, Dingqian [2 ]
Hou, Huomei [2 ]
Bulmer, Judith N. [1 ]
Innes, Barbara A. [1 ]
Hapangama, Dharani K. [3 ]
Lash, Gendie E. [2 ]
机构
[1] Newcastle Univ, Inst Cellular Med, Reprod & Vasc Biol Grp, Newcastle Upon Tyne NE2 4HH, England
[2] Guangzhou Med Univ, Guangzhou Inst Pediat, Guangzhou Women & Childrens Med Ctr, Div Uterine Vasc Biol, Guangzhou 510623, Peoples R China
[3] Univ Liverpool, Liverpool Womens Hosp, Inst Life Course & Med Sci, Dept Womens & Childrens Hlth, Crown St, Liverpool L8 7SS, England
基金
中国国家自然科学基金;
关键词
Abnormal uterine bleeding; Endometrium; Endothelial cells; Platelet-derived growth factor BB; VSMC differentiation; MENSTRUAL-CYCLE; IN-VITRO; BETA; ANGIOGENESIS; PROLIFERATION; EXPRESSION; RECEPTORS; PDGF; LOCALIZATION; DIFFERENTIATION;
D O I
10.1016/j.rbmo.2022.02.005
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Research question: What is the expression pattern of platelet-derived growth factor BB (PDGF-BB), and its receptors, across the menstrual cycle in healthy control women and those with abnormal uterine bleeding-endometrial disorder (AUB-E)? Design: Immunohistochemical staining for PDGF-BB, platelet-derived growth factor receptor alpha (PDGFR alpha) and platelet-derived growth factor beta (PDGFR beta) was performed in control and AUB-E endometrium from the proliferative, early, mid- and late secretory phases of the menstrual cycle (n = 5 each group). Control proliferative phase endometrium was cultured in PDGF-BB (0, 10 ng/ml) and vascular maturation assessed (n = 3). Endothelial cell to vascular smooth muscle cell (VSMC) association was assessed after treatment with PDGF-BB (0, 1, 10 ng/ml). Secretion of angiogenic growth factors by endothelial cells or VSMC was determined. Results: Endothelial cell immunoreactivity for PDGF-BB was reduced in the mid and late secretory phases in AUB-E (P = 0.008). PDGFR alpha was also reduced in mid secretory phase endothelial cells, proliferative and early secretory phase glandular epithelium in AUB-E (P = 0.008). PDGFR beta expression was not altered. Treatment of proliferative phase endometrium with PDGF-BB (10 ng/ml) reduced the percentage of vessels expressing contractile VSMC markers. PDGF-BB had no effect on angiogenic growth factor secretion by endothelial cells or VSMC in vitro and did not affect their association in an in-vitro endothelial cell-VSMC association assay. Conclusions: Reduced endothelial cell expression of PDGF-BB in the AUB-E endometrium may contribute to the reduced vascular maturation previously observed in these women.
引用
收藏
页码:531 / 543
页数:13
相关论文
共 50 条
[41]   Serum levels of platelet-derived growth factor-BB and vascular endothelial growth factor as prognostic factors for patients with fulminant hepatic failure [J].
Takayama, Hiroki ;
Miyake, Yasuhiro ;
Nouso, Kazuhiro ;
Ikeda, Fusao ;
Shiraha, Hidenori ;
Takaki, Akinobu ;
Kobashi, Haruhiko ;
Yamamoto, Kazuhide .
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2011, 26 (01) :116-121
[42]   Endometrial Sampling Results of Women Evaluated for Abnormal Uterine Bleeding: A Retrospective Analysis of 765 Women [J].
Aker, Seda Sahin ;
Yuce, Tuncay ;
Acar, Dilek ;
Atabekoglu, Cem Somer .
CUKUROVA MEDICAL JOURNAL, 2015, 40 (02) :306-310
[43]   Platelet-derived growth factor BB secreted from osteoclasts acts as an osteoblastogenesis inhibitory factor [J].
Kubota, K ;
Sakikawa, C ;
Katsumata, M ;
Nakamura, T ;
Wakabayashi, K .
JOURNAL OF BONE AND MINERAL RESEARCH, 2002, 17 (02) :257-265
[44]   Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation [J].
Yi-Jin Tao ;
Qin Chen ;
Li Wang ;
Xiao Yang ;
Qing Cun ;
Wen-Yan Yang ;
Hua Zhong .
International Journal of Ophthalmology, 2019, 12 (07) :1075-1082
[45]   Hypoxia promotes osteosarcoma cell proliferation and migration through enhancing platelet-derived growth factor-BB/platelet-derived growth factor receptor-β axis [J].
Zhang, Dan ;
Cui, Gang ;
Sun, Chao ;
Lei, Li ;
Lei, Lei ;
Williamson, Ramone A. ;
Wang, Yanmeng ;
Zhang, Juan ;
Chen, Ping ;
Wang, Aiying ;
Fan, Rong ;
Han, Suxia ;
Wang, Yili ;
Hu, Jinsong .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 512 (02) :360-366
[46]   Accuracy of endometrial sampling compared to conventional dilatation and curettage in women with abnormal uterine bleeding [J].
Abdelazim, Ibrahim A. ;
Abdelrazak, Khaled M. ;
Elbiaa, Assem A. M. ;
Al-Kadi, Mohamed ;
Yehia, Amr H. .
ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 2015, 291 (05) :1121-1126
[47]   Endometrial Ablation in Women With Abnormal Uterine Bleeding Related to Ovulatory Dysfunction: A Cohort Study [J].
Hokenstad, Alexis N. ;
El-Nashar, Sherif A. ;
Khan, Zaraq ;
Hopkins, Matthew R. ;
Famuyide, Abimbola O. .
JOURNAL OF MINIMALLY INVASIVE GYNECOLOGY, 2015, 22 (07) :1225-1230
[48]   Optimal Timing of Transvaginal Ultrasound to Diagnose Endometrial Polyps in Women with Abnormal Uterine Bleeding [J].
Jahromi, Athar Rasekh ;
Ebadat, Hamideh ;
Jokar, Mohammad ;
Taghipour, Nikta ;
Rahmanian, Vahid .
JOURNAL OF OBSTETRICS AND GYNECOLOGY OF INDIA, 2025, 75 (SUPPL1) :62-69
[49]   Platelet-derived growth factor-AA is a substantial factor in the ability of adipose-derived stem cells and endothelial progenitor cells to enhance wound healing [J].
Wu, Li-Wei ;
Chen, Wei-Liang ;
Huang, Shih-Ming ;
Chan, James Yi-Hsin .
FASEB JOURNAL, 2019, 33 (02) :2388-2395
[50]   Artemisinin attenuates platelet-derived growth factor BB-induced migration of vascular smooth muscle cells [J].
Lee, Kang Pa ;
Park, Eun-Seok ;
Kim, Dae-Eun ;
Park, In-Sik ;
Kim, Jin Tack ;
Hong, Heeok .
NUTRITION RESEARCH AND PRACTICE, 2014, 8 (05) :521-525