LARP7 family proteins have conserved function in telomerase assembly

被引：34

作者：

Collopy, Laura C. ^{[1
]}

Ware, Tracy L. ^{[1
,2
]}

Goncalves, Tomas ^{[1
,3
]}

Sunnvor i Kongsstovu ^{[1
,4
]}

Yang, Qian ^{[1
]}

Amelina, Hanna ^{[1
]}

Pinder, Corinne ^{[1
,3
]}

Alenazi, Ala ^{[1
,4
]}

Moiseeva, Vera ^{[1
]}

Pearson, Sian R. ^{[1
]}

Armstrong, Christine A. ^{[1
]}

Tomita, Kazunori ^{[1
]}

机构：

[1] UCL, UCL Canc Inst, Chromosome Maintenance Grp, London WC1E 6DD, England

[2] Salem State Univ, Dept Biol, Salem, MA 01970 USA

[3] UCL, Fac Life Sci, Div Biosci, London WC1E 6BT, England

[4] Imperial Coll London, Fac Med, MSc Human Mol Genet, London SW7 2AZ, England

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

欧洲研究理事会;

关键词：

C-TERMINAL DOMAIN; FISSION YEAST; RNA-BINDING; DYSKERATOSIS-CONGENITA; BIOGENESIS; HOLOENZYME; CANCER; P65; RECRUITMENT; DEFICIENCY;

D O I：

10.1038/s41467-017-02296-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Understanding the intricacies of telomerase regulation is crucial due to the potential health benefits of modifying its activity. Telomerase is composed of an RNA component and reverse transcriptase. However, additional factors required during biogenesis vary between species. Here we have identified fission yeast Lar7 as a member of the conserved LARP7 family, which includes the Tetrahymena telomerase-binding protein p65 and human LARP7. We show that Lar7 has conserved RNA-recognition motifs, which bind telomerase RNA to protect it from exosomal degradation. In addition, Lar7 is required to stabilise the association of telomerase RNA with the protective complex LSm2-8, and telomerase reverse transcriptase. Lar7 remains a component of the mature telomerase complex and is required for telomerase localisation to the telomere. Collectively, we demonstrate that Lar7 is a crucial player in fission yeast telomerase biogenesis, similarly to p65 in Tetrahymena, and highlight the LARP7 family as a conserved factor in telomere maintenance.

引用

页数：8

共 51 条

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