Study on the interaction between aglycon of daunorubicin and calf thymus DNA by spectroscopy

The interaction between aglycon of daunorubicin (DNR-A) and calf thymus DNA (ctDNA) was investigated by UV absorption spectra and fluorescence spectra. Hypochromic effect was founded in the absorption spectra of DNR-A in the presence of ctDNA. The fluorescence intensity of DNR-A was decreased with the addition of ctDNA. Stern-Volmer plots at different temperatures proved that the quenching mechanism was a static quenching type. The binding constants of DNR-A with ctDNA at 301, 310 and 320 K were 6.203, 4.782, 3.334 x 10(4) L mol(-1), respectively. The thermodynamic parameters were also obtained, which suggested that hydrophobic force might play a major role in the binding of DNR-A to ctDNA. The relative viscosity of ctDNA increased with the addition of DNR-A, at the same time the value of melting temperature of ctDNA increased in the presence of DNR-A. Effect of ionic strength showed that the addition of NaCl could not affect the binding of DNR-A and ctDNA. In addition, fluorescence quenching study indicated that K-SV value for the bound DNR-A with ctDNA was lower than the free DNR-A. Fluorescence polarization and denatured DNA studies also applied strong evidences that DNR-A molecule was intercalated between the base pairs of ctDNA. All the results supported that the major binding mode of DNR-A and ctDNA was intercalation. These studies were helpful for a better understanding in the interaction of DNR-A with ctDNA, which should be beneficial to the design of new DNA targeted drugs. (C) 2011 Elsevier B.V. All rights reserved.