Functional Role of γ-Crystallin N in the Auditory Hindbrain

被引:6
作者
Hartwich, Heiner [1 ]
Rosengauer, Elena [1 ]
Ruettiger, Lukas [2 ]
Wilms, Viviane [1 ]
Waterholter, Sarah-Kristin [1 ]
Nothwang, Hans Gerd [1 ,3 ]
机构
[1] Carl von Ossietzky Univ Oldenburg, Sch Med & Hlth Sci, Neurogenet Grp, Ctr Excellence Hearing4All, D-26111 Oldenburg, Germany
[2] Univ Tubingen, Dept Otolaryngol, THRC, Mol Physiol Hearing, Elfriede Aulhorn Str 5, D-72076 Tubingen, Germany
[3] Carl von Ossietzky Univ Oldenburg, Res Ctr Neurosensory Sci, D-26111 Oldenburg, Germany
来源
PLOS ONE | 2016年 / 11卷 / 08期
关键词
GLYCINE TRANSPORTER GLYT2; BRAIN-STEM; COCHLEAR NUCLEUS; MEDIAL NUCLEUS; EXPRESSION; GENE; EVOLUTION; PROTEINS; PATTERN; HEARING;
D O I
10.1371/journal.pone.0161140
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
gamma-crystallins are major components of the vertebrate lens but show expression in other tissues as well. Their extralenticular functions remain so far unclear. Here, we explored such roles in the rodent superior olivary complex in which previous analysis demonstrated developmentally regulated expression of Crygd, Cryge and Crygn. Immunohistochemistry with novel antibodies against Crygd/e and Crygn indicate that expression of Crygd/e was moderate and varied between the perinatal superior olivary complex of mice, rats, and gerbils. Crygn-immunoreactivity was more robust and consistently highest in the medial nucleus of the trapezoid body, but also present in other nuclei of the superior olivary complex. To analyze the function of Crygn in the auditory hindbrain, we used a Crygn allele with a floxed exon 2. Upon pairing with Egr2::Cre mice, exon 2, encoding the first two greek key motifs of Crygn, was deleted in the developing auditory hindbrain. Anatomical analysis of these mice revealed a 20% volume reduction in the medial nucleus of the trapezoid body and a 7% reduction in the lateral superior olive at postnatal day 25. This was due to cell loss between postnatal days 4 and 25, whereas cell size was unaffected. Auditory brainstem responses showed normal threshold but a significant increase in the amplitude of wave IV. Crygn is hence required for postmigratory survival and proper function of auditory hindbrain neurons. These results ascertain for the first time an essential extralenticular role for gamma-crystallins in vivo.
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页数:22
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