Digital pathology with Fourier ptychography

被引：104

作者：

Horstmeyer, Roarke ^{[1
]}

Ou, Xiaoze ^{[1
]}

Zheng, Guoan ^{[2
]}

Willems, Phil ^{[3
]}

Yang, Changhuei ^{[1
]}

机构：

[1] CALTECH, Dept Elect Engn & Bioengn, Pasadena, CA 91125 USA

[2] Univ Connecticut, Dept Biomed Engn, Storrs, CT 06268 USA

[3] ePetri Inc, Pasadena, CA 91106 USA

来源：

COMPUTERIZED MEDICAL IMAGING AND GRAPHICS | 2015年 / 42卷

基金：

美国国家卫生研究院;

关键词：

Digital pathology; Computational imaging; Structured illumination; Super-resolution; Microscopy; SCATTERING-PHASE THEOREM; HIGH-RESOLUTION; WIDE-FIELD; MICROSCOPY;

D O I：

10.1016/j.compmedimag.2014.11.005

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Fourier ptychographic microscopy (FPM) is a recently introduced method of acquiring high-resolution, wide field of view (FOV) giga-pixel histology images. The FPM procedure first acquires a sequence of low-resolution images of a sample under variable-angle illumination. It then combines these images using a novel phase retrieval algorithm to improve die employed microscope's resolution beyond its conventional limit. Here, we first describe how FPM's resolution improvement can enhance wide FOV histology imaging. Second, we show that FPM also records a thin sample's optical phase, which can help pathologists digitally extract as much information as possible from a given histology slide. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：38 / 43

页数：6

共 19 条

[1] Multiscale gigapixel photography [J].

Brady, D. J. ;

Gehm, M. E. ;

Stack, R. A. ;

Marks, D. L. ;

Kittle, D. S. ;

Golish, D. R. ;

Vera, E. M. ;

Feller, S. D. .

NATURE, 2012, 486 (7403) :386-389

[2] Increased space-bandwidth product in pixel super-resolved lensfree on-chip microscopy [J].

Greenbaum, Alon ;

Luo, Wei ;

Khademhosseinieh, Bahar ;

Su, Ting-Wei ;

Coskun, Ahmet F. ;

Ozcan, Aydogan .

SCIENTIFIC REPORTS, 2013, 3

[3] High-resolution, wide-field object reconstruction with synthetic aperture Fourier holographic optical microscopy [J].

Hillman, Timothy R. ;

Gutzler, Thomas ;

Alexandrov, Sergey A. ;

Sampson, David D. .

OPTICS EXPRESS, 2009, 17 (10) :7873-7892

[4] A phase space model of Fourier ptychographic microscopy [J].

Horstmeyer, Roarke ;

Yang, Changhuei .

OPTICS EXPRESS, 2014, 22 (01) :338-358

[5] SCALING LAWS FOR LENS SYSTEMS [J].

LOHMANN, AW .

APPLIED OPTICS, 1989, 28 (23) :4996-4998

[6] Synthetic aperture superresolution with multiple off-axis holograms [J].

Mico, Vicente ;

Zalevsky, Zeev ;

Garcia-Martinez, Pascuala ;

Garcia, Javier .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2006, 23 (12) :3162-3170

[7] Embedded pupil function recovery for Fourier ptychographic microscopy [J].

Ou, Xiaoze ;

Zheng, Guoan ;

Yang, Changhuei .

OPTICS EXPRESS, 2014, 22 (05) :4960-4972

[8] Quantitative phase imaging via Fourier ptychographic microscopy [J].

Ou, Xiaoze ;

Horstmeyer, Roarke ;

Yang, Changhuei ;

Zheng, Guoan .

OPTICS LETTERS, 2013, 38 (22) :4845-4848

[9]

Popescu G., 2011, Quantitative Phase Imaging of Cells and Tissues

[10]

Prahl S, 2012, INTERACTIVE MIE CALC

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