Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue

被引：5
|
作者：
Saraji, Alireza [1 ]
Offermann, Anne [1 ]
Stegmann-Frehse, Janine [1 ]
Hempel, Katharina [1 ]
Kang, Duan [1 ]
Krupar, Rosemarie [2 ]
Watermann, Christian [1 ]
Jonigk, Danny [3 ,4 ]
Kuehnel, Mark Philipp [3 ,4 ]
Kirfel, Jutta [1 ]
Perner, Sven [1 ,2 ]
Sailer, Verena [1 ]
机构：
[1] Univ Hosp Schleswig Holstein, Pathol, Campus Luebeck, Lubeck, Germany
[2] Leibniz Lung Ctr, Res Ctr Borstel, Borstel, Germany
[3] Hannover Med Sch, Inst Pathol, Hannover, Germany
[4] German Ctr Lung Res, Biomed Res Endstage & Obstruct Lung Dis BREATH, Hannover, Germany
来源：
PLOS ONE | 2021年 / 16卷 / 09期
关键词：
PROSTATE-CANCER; METASTASES; OPTIMIZATION; PURIFICATION;
D O I：
10.1371/journal.pone.0257416
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
With the advance of precision medicine, the availability of tumor tissue for molecular analysis has become a limiting factor. This is particularly the case for bone metastases which are frequently occurring in cancer types such as prostate cancer. Due to the necessary decalcification process it was long thought that transcriptome analysis will not be feasible from decalcified formalin-fixed, paraffin-embedded (DFFPE) in a large manner. Here we demonstrate that mRNA extraction from DFFPE is feasible, quick, robust and reproducible and that decalcification does not hamper subsequent gene expression analysis. This might assist in implementing transcriptome analysis from DFFPE into every day practice.
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页数：11
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