STD and trNOESY NMR Study of Receptor-Ligand Interactions in Living Cancer Cells

被引：33

作者：

Potenza, Donatella ^{[1
]}

Vasile, Francesca ^{[2
]}

Belvisi, Laura ^{[1
]}

Civera, Monica ^{[2
]}

Araldi, Elena M. V. ^{[2
]}

机构：

[1] Univ Milan, Dipartimento Chim Organ & Ind, Ctr Interdipartimentale CISI, Ist Sci & Tecnol Mol,CNR, I-20133 Milan, Italy

[2] Univ Milan, Ctr Interdipartimentale CISI, I-20138 Milan, Italy

来源：

CHEMBIOCHEM | 2011年 / 12卷 / 05期

关键词：

living cancer cells; membrane proteins; molecular modeling; protein-ligand interactions; STD-NMR; RGD; INTEGRINS; ALPHA-V-BETA-3; PLATELETS; PROTEINS; BINDING; MIMICS; ACIDS;

D O I：

10.1002/cbic.201000756

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Integrin antagonists: Saturation transfer difference (STD) and transferred NOE provided useful information on membrane-bound receptor-ligand interactions in living cells in which the integrin αvβ3 is highly expressed. The epitopes of two cyclic RGD mimics were identified by STD. The different activities of these compounds were interpreted in terms of different binding modes. The experimental evidence was confirmed by docking calculations. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：695 / 699

页数：5