miRNA Profiling of Hungarian Regressive Wilms' Tumor Formalin-Fixed Paraffin-Embedded (FFPE) Samples by Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

被引:7
作者
Buglyo, Gergely [1 ]
Magyar, Zsofia [2 ]
Gorbe, Eva Romicsne [2 ]
Banusz, Rita [3 ]
Csoka, Monika [3 ]
Micsik, Tamas [4 ]
Mezei, Marta [1 ]
Yani, Jaxi Ayman Shawky [1 ]
Varga, Peter [2 ]
Sapi, Zoltan [4 ]
Nagy, Balint [1 ]
机构
[1] Univ Debrecen, Fac Med, Dept Human Genet, Debrecen, Hungary
[2] Semmelweis Univ, Dept Obstet & Gynaecol, Baross St Div, Budapest, Hungary
[3] Semmelweis Univ, Dept Paediat 2, Budapest, Hungary
[4] Semmelweis Univ, Dept Pathol & Expt Canc Res 1, Budapest, Hungary
来源
MEDICAL SCIENCE MONITOR | 2021年 / 27卷
关键词
MIRN184; microRNA; Human; MIRN194; MIRN203; Real-Time Polymerase Chain Reaction; Wilms Tumor; EXPRESSION; PROLIFERATION; APOPTOSIS; MIR-184;
D O I
10.12659/MSM.932731
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Background: Wilms' tumor is a common renal malignancy of early childhood with a generally favorable prognosis depending upon histological subtype. It is becoming increasingly clear that differences in miRNA (microRNA) expression signature represent important clues helping us predict a tumor's response to chemotherapy. In our study, we aimed to reveal miRNAs deregulated in regressive Wilms' tumors from FFPE (formalin-fixed, paraffin -embedded) samples, also showing whether such samples are reliable miRNA sources in Wilms' tumor. Material/Methods: Samples from 8 Hungarian patients (3 males, 5 females, aged 1 to 7 years) were analyzed by qRT-PCR (quantitative real-time PCR). A PCR array was used in a pilot experiment, and selected miRNAs (miR-128-3p, miR-184, miR-194-5p, miR-203a) were studied in the rest of the samples using individual primers. Results: miR-194-5p was underexpressed in all tumor samples. miR-184 and miR-203a were underexpressed in 7 cases, the exception being a case with a high ratio of necrotic blastemal tissue. Results obtained with miR-1283p are difficult to interpret due to varying directions of expression changes. Conclusions: We conclude that a downregulation of miR-184, miR-194-5p, and miR-203a expression is observed in both regressive and blastemal tumors, but larger-scale studies are needed to confirm whether the degree of their underexpression correlates with the number of blastemal elements in a sample. In most of our FFPE samples aged up to 9 years, RNA extraction provided miRNA with quantity and quality sufficient for qRT-PCR-based analysis, emphasizing the relevance of pathological archives as miRNA sources in future studies.
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页数:9
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