ENGINEERED GREEN FLUORESCENT PROTEIN AS A POTENTIAL METAL SENSOR

被引：0

作者：

Maria, Palfi ^{[1
]}

Erika, Kovacs ^{[1
]}

Ildiko, Miklossy ^{[2
]}

Laszlo, Szilagyi ^{[2
,3
]}

Beata, Abraham ^{[2
]}

Szabolcs, Lanyi ^{[2
]}

机构：

[1] Univ Politehn Bucuresti, Fac Chim Aplicata Si Stiinta Mat, RO-060042 Bucharest, Romania

[2] Univ Sapientia Cluj Napoca, Fac Stiinte Tehnice Si Sociale, Catedra Stiinte Tehnice Si Ale Nat, RO-5301104 Miercurea Ciuc, Romania

[3] Eotvos Lorand Univ, Dept Biochem, H-1117 Budapest, Hungary

来源：

STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA | 2009年 / 54卷

关键词：

green fluorescent protein; metal binding; site-specific mutation; chromophore group; biosensor; BIOSENSOR APPLICATIONS; GFP; DENATURATION;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The designed biosensor is based on a mutant form of green fluorescent protein (GFP). It is known that metal ions in close proximity to a cromophore group reduce the protein' s fluorescence intensity by fluorescence quenching. Using site directed mutagenesis, we formed a metal binding site near the protein's chromophore group. Thus, the mutant protein presents a new functional property, which allows its utilization in detection of metal ions.

引用

页码：35 / 43

页数：9

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