ENGINEERED GREEN FLUORESCENT PROTEIN AS A POTENTIAL METAL SENSOR

被引：0
作者：
Maria, Palfi [1 ]
Erika, Kovacs [1 ]
Ildiko, Miklossy [2 ]
Laszlo, Szilagyi [2 ,3 ]
Beata, Abraham [2 ]
Szabolcs, Lanyi [2 ]
机构：
[1] Univ Politehn Bucuresti, Fac Chim Aplicata Si Stiinta Mat, RO-060042 Bucharest, Romania
[2] Univ Sapientia Cluj Napoca, Fac Stiinte Tehnice Si Sociale, Catedra Stiinte Tehnice Si Ale Nat, RO-5301104 Miercurea Ciuc, Romania
[3] Eotvos Lorand Univ, Dept Biochem, H-1117 Budapest, Hungary
来源：
STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA | 2009年 / 54卷
关键词：
green fluorescent protein; metal binding; site-specific mutation; chromophore group; biosensor; BIOSENSOR APPLICATIONS; GFP; DENATURATION;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The designed biosensor is based on a mutant form of green fluorescent protein (GFP). It is known that metal ions in close proximity to a cromophore group reduce the protein' s fluorescence intensity by fluorescence quenching. Using site directed mutagenesis, we formed a metal binding site near the protein's chromophore group. Thus, the mutant protein presents a new functional property, which allows its utilization in detection of metal ions.
引用
收藏
页码：35 / 43
页数：9
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