Sperm Vitality and Necrozoospermia: Diagnosis, Management, and Results of a Global Survey of Clinical Practice

被引：23

作者：

Agarwal, Ashok ^{[1
]}

Sharma, Rakesh K. ^{[1
]}

Gupta, Sajal ^{[1
]}

Boitrelle, Florence ^{[2
,3
]}

Finelli, Renata ^{[1
]}

Parekh, Neel ^{[4
]}

Durairajanayagam, Damayanthi ^{[5
]}

Saleh, Ramadan ^{[6
]}

Arafa, Mohamed ^{[1
,7
,8
]}

Cho, Chak Lam ^{[9
]}

Farkouh, Ala'a ^{[1
]}

Rambhatla, Amarnath ^{[10
]}

Henkel, Ralf ^{[1
,11
,12
,13
]}

Vogiatzi, Paraskevi ^{[14
]}

Tadros, Nicholas ^{[15
]}

Kavoussi, Parviz ^{[16
]}

Ko, Edmund ^{[17
]}

Leisegang, Kristian ^{[18
]}

Kandil, Hussein ^{[19
]}

Palani, Ayad ^{[20
]}

Salvio, Gianmaria ^{[21
]}

Mostafa, Taymour ^{[22
]}

Rajmil, Osvaldo ^{[23
]}

Banihani, Saleem Ali ^{[24
]}

Schon, Samantha ^{[25
]}

Le, Tan V. ^{[26
,27
]}

Birowo, Ponco ^{[28
]}

Ceker, Gokhan ^{[29
]}

Alvarez, Juan ^{[30
]}

Molina, Juan Manuel Corral ^{[31
]}

Ho, Christopher C. K. ^{[32
]}

Calogero, Aldo E. ^{[33
]}

Khalafalla, Kareim ^{[7
]}

Duran, Mesut Berkan ^{[34
]}

Kuroda, Shinnosuke ^{[1
]}

Colpi, Giovanni M. ^{[35
]}

Zini, Armand ^{[36
]}

Anagnostopoulou, Christina ^{[37
]}

Pescatori, Edoardo ^{[38
]}

Chung, Eric ^{[39
,40
]}

Caroppo, Ettore ^{[41
,43
]}

Dimitriadis, Fotios ^{[42
]}

Pinggera, Germar-Michael

Busetto, Gian Maria ^{[44
]}

Balercia, Giancarlo ^{[21
]}

Elbardisi, Haitham ^{[7
,8
]}

Taniguchi, Hisanori ^{[45
]}

Park, Hyun Jun ^{[46
,47
]}

Rosas, Israel Maldonado ^{[48
]}

de la Rosette, Jean ^{[49
]}

机构：

[1] Cleveland Clin, Amer Ctr Reprod Med, Mail Code X-11,10681 Carnegie Ave, Cleveland, OH 44195 USA

[2] Poissy Hosp, CECOS, Reprod Biol, Fertil,Preservat,Androl, Poissy, France

[3] Univ Paris Saclay, UVSQ, INRAE, BREED, Paris, France

[4] Cleveland Clin, Dept Urol, Cleveland, OH 44106 USA

[5] Univ Teknol MARA, Fac Med, Dept Physiol, Sungai Buloh Campus, Shah Alam, Selangor, Malaysia

[6] Sohag Univ, Fac Med, Dept Dermatol Venereol & Androl, Sohag, Egypt

[7] Hamad Med Corp, Dept Urol, Doha, Qatar

[8] Weill Cornell Med Qatar, Dept Urol, Doha, Qatar

[9] Chinese Univ Hong Kong, Dept Surg, SH Ho Urol Ctr, Hong Kong, Peoples R China

[10] Henry Ford Hlth Syst, Vattikuti Urol Inst, Dept Urol, Detroit, MI USA

[11] Imperial Coll London, Dept Metab Digest & Reprod, London, England

[12] Univ Western Cape, Dept Med Biosci, Bellville, South Africa

[13] LogixX Pharma, Theale, Berks, England

[14] Fertil Diagnost Lab, Andromed Hlth Reprod, Maroussi, Greece

[15] Southern Illinois Univ, Div Urol, Sch Med, Springfield, IL USA

[16] Univ Texas Hlth Sci Ctr San Antonio, Dept Urol, Austin Fertil & Reprod Med Westlake IVF, San Antonio, TX 78229 USA

[17] Loma Linda Univ Hlth, Dept Urol, Loma Linda, CA USA

[18] Univ Western Cape, Fac Community & Hlth Sci, Sch Nat Med, Dept Physiol, Bellville, South Africa

[19] Fakih IVF Fertil Ctr, Abu Dhabi, U Arab Emirates

[20] Univ Garmian, Coll Med, Dept Biochem, Kalar, Iraq

[21] Polytech Univ Marche, Dept Endocrinol & Metab Dis, Ancona, Italy

[22] Cairo Univ, Fac Med, Dept Androl Sexol & STIs, Cairo, Egypt

[23] Fundacio Puigvert, Dept Androl, Barcelona, Spain

[24] Jordan Univ Sci & Technol, Fac Appl Med Sci, Dept Med Lab Sci, Irbid, Jordan

[25] Univ Michigan, Dept Obstet & Gynecol, Div Reprod Endocrinol & Infertil, Ann Arbor, MI 48109 USA

[26] Pham Ngoc Thach Univ Med, Dept Androl & Urol, Ho Chi Minh City, Vietnam

[27] Binh Dan Hosp, Dept Androl, Ho Chi Minh City, Vietnam

[28] Univ Indonesia, Cipto Mangunkusumo Gen Hosp, Dept Urol, Fac Med, Jakarta, Indonesia

[29] Samsun Vezirkopru State Hosp, Dept Urol, Samsun, Turkey

[30] Ctr ANDROGEN, La Coruna, Spain

[31] Hosp Clin Barcelona, Serv Urol, Barcelona, Spain

[32] Taylors Univ, Sch Med, Fac Hlth & Med Sci, Dept Surg, Subang Jaya, Malaysia

[33] Univ Catania, Dept Clin & Expt Med, Catania, Italy

[34] Samsun Training & Res Hosp, Dept Urol, Samsun, Turkey

[35] Procrea Inst, Androl & IVF Unit, Lugano, Switzerland

[36] McGill Univ, Dept Surg, Montreal, PQ, Canada

[37] IVF Clin Akeso Embryo ART, Athens, Greece

[38] Gynepro Med, Androl & Reprod Med Unit, Bologna, Italy

[39] Univ Queensland, Princess Alexandra Hosp, Dept Urol, Brisbane, Qld, Australia

[40] AndroUrol Ctr, Brisbane, Qld, Australia

[41] ASL Bari, Androl Outpatients Clin, Reprod & IVF Unit, Conversano, Ba, Italy

[42] Aristotle Univ Thessaloniki, Sch Med, Dept Urol 1, Thessaloniki, Greece

[43] Med Univ Innsbruck, Dept Urol, Innsbruck, Austria

[44] Univ Foggia, Dept Urol & Renal Transplantat, Osped Riuniti Foggia, Foggia, Italy

[45] Kansai Med Univ, Dept Urol & Androl, Hirakata, Osaka, Japan

[46] Pusan Natl Univ, Sch Med, Dept Urol, Busan, South Korea

[47] Pusan Natl Univ Hosp, Med Res Inst, Busan, South Korea

[48] IVF LAB, Citmer Reprod Med, Mexico City, DF, Mexico

[49] Medipol Mega Univ Hosp, Dept Urol, Istanbul, Turkey

[50] Hammersmith Hosp, Dept Androl, London, England

来源：

WORLD JOURNAL OF MENS HEALTH | 2022年 / 40卷 / 02期

关键词：

Asthenozoospermia; EosineYellowish-(YS); Infertility; Nigrosin; Spermatozoa; Vitality; HYPOOSMOTIC SWELLING TEST; MALE-INFERTILITY; TESTICULAR SPERMATOZOA; SEMINAL PLASMA; SEMEN QUALITY; DNA-DAMAGE; IMMOTILE; ICSI; PARAMETERS; VIABILITY;

D O I：

10.5534/wjmh.210149

中图分类号：

R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

摘要：

Sperm vitality testing is a basic semen examination that has been described in the World Health Organization (WHO) Laboratory Manual for the Examination and Processing of Human Semen from its primary edition, 40 years ago. Several methods can be used to test sperm vitality, such as the eosin-nigrosin (E-N) stain or the hypoosmotic swelling (HOS) test. In the 6th (2021) edition of the WHO Laboratory Manual, sperm vitality assessment is mainly recommended if the total motility is less than 40%. Hence, a motile spermatozoon is considered alive, however, in certain conditions an immotile spermatozoon can also be alive. Therefore, the differentiation between asthenozoospermia (pathological decrease in sperm motility) and necrozoospermia (pathological decrease in sperm vitality) is important in directing further investigation and management of infertile patients. The causes leading to necrozoospermia are diverse and can either be local or general, testicular or extra -testicular. The andrological management of necrozoospermia depends on its etiology. However, there is no standardized treatment available presently and practice varies among clinicians. In this study, we report the results of a global survey to understand current practices regarding the physician order of sperm vitality tests as well as the management practices for necrozoospermia. Laboratory and clinical scenarios are presented to guide the reader in the management of necrozoospermia with the overall objective of establishing a benchmark ranging from the diagnosis of necrozoospermia by sperm vitality testing to its clinical management.

引用

页码：228 / 242

页数：15

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