Transcriptomic analysis of genes related to alkaloid biosynthesis and the regulation mechanism under precursor and methyl jasmonate treatment in Dendrobium officinale

被引:14
|
作者
Jiao, Chunyan [1 ,2 ]
Wei, Mengke [2 ]
Fan, Honghong [1 ]
Song, Cheng [3 ]
Wang, Zhanjun [2 ,4 ]
Cai, Yongping [1 ]
Jin, Qing [1 ]
机构
[1] Anhui Agr Univ, Sch Life Sci, Hefei, Peoples R China
[2] Hefei Normal Univ, Coll Life Sci, Hefei, Peoples R China
[3] West Anhui Univ, Coll Biol & Pharmaceut Engn, Luan, Peoples R China
[4] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha, Peoples R China
来源
关键词
Dendrobium officinale; alkaloid biosynthesis; precursors; methyl jasmonate; transcriptome; INDOLE ALKALOIDS; EXPRESSION; SYNTHASE;
D O I
10.3389/fpls.2022.941231
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Dendrobium officinale is both a traditional herbal medicine and a plant of high ornamental and medicinal value. Alkaloids, especially terpenoid indole alkaloids (TIAs), with pharmacological activities are present in the tissues of D. officinale. A number of genes involved in alkaloid biosynthetic pathways have been identified. However, the regulatory mechanisms underlying the precursor and methyl jasmonate (MeJA)-induced accumulation of alkaloids in D. officinale are poorly understood. In this study, we collected D. officinale protocorm-like bodies (PLBs) and treated them with TIA precursors (tryptophan and secologanin) and MeJA for 0 (T0), 4 (T4) and 24 h (T24); we also established control samples (C4 and C24). Then, we measured the total alkaloid content of the PLBs and performed transcriptome sequencing using the Illumina HiSeq 2,500 system. The total alkaloid content increased significantly after 4 h of treatment. Go and KEGG analysis suggested that genes from the TIA, isoquinoline alkaloid, tropane alkaloid and jasmonate (JA) biosynthetic pathways were significantly enriched. Weighted gene coexpression network analysis (WGCNA) uncovered brown module related to alkaloid content. Six and seven genes related to alkaloid and JA bisosynthetic pathways, respectively, might encode the key enzymes involved in alkaloid biosynthesis of D. officinale. Moreover, 13 transcription factors (TFs), which mostly belong to AP2/ERF, WRKY, and MYB gene families, were predicted to regulate alkaloid biosynthesis. Our data provide insight for studying the regulatory mechanism underlying TIA precursor and MeJA-induced accumulation of three types of alkaloids in D. officinale.
引用
收藏
页数:17
相关论文
共 21 条
  • [1] Transcriptomic and Metabolomic Analysis Unravels the Molecular Regulatory Mechanism of Fatty Acid Biosynthesis in Styrax tonkinensis Seeds under Methyl Jasmonate Treatment
    Chen, Chen
    Chen, Hong
    Han, Chao
    Liu, Zemao
    Ni, Ming
    Wu, Qikui
    Yu, Fangyuan
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (11)
  • [2] Characterization of the WRKY Gene Family Related to Anthocyanin Biosynthesis and the Regulation Mechanism under Drought Stress and Methyl Jasmonate Treatment in Lycoris radiata
    Wang, Ning
    Song, Guowei
    Zhang, Fengjiao
    Shu, Xiaochun
    Cheng, Guanghao
    Zhuang, Weibing
    Wang, Tao
    Li, Yuhang
    Wang, Zhong
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023, 24 (03)
  • [3] Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale
    Chen, Yue
    Wang, Yunzhu
    Lyu, Ping
    Chen, Liping
    Shen, Chenjia
    Sun, Chongbo
    JOURNAL OF PLANT RESEARCH, 2019, 132 (03) : 419 - 429
  • [4] Comparative transcriptomic analysis reveal the regulation mechanism underlying MeJA-induced accumulation of alkaloids in Dendrobium officinale
    Yue Chen
    Yunzhu Wang
    Ping Lyu
    Liping Chen
    Chenjia Shen
    Chongbo Sun
    Journal of Plant Research, 2019, 132 : 419 - 429
  • [5] Transcriptome analysis reveals regulation mechanism of methyl jasmonate-induced terpenes biosynthesis in Curcuma wenyujin
    Wei, Qiuhui
    Lan, Kaer
    Liu, Yuyang
    Chen, Rong
    Hu, Tianyuan
    Zhao, Shujuan
    Yin, Xiaopu
    Xie, Tian
    PLOS ONE, 2022, 17 (06):
  • [6] Transcriptomic Analysis Reveals the Biosynthesis Mechanism of Coixol Under Salicylic Acid Treatment
    Wang, Yao
    Ye, Hanli
    Luo, Xuqin
    Li, Ziwei
    Zheng, Chuanqi
    Sun, Dali
    HORTICULTURAE, 2025, 11 (03)
  • [7] Full-length transcriptome sequencing and methyl jasmonate-induced expression profile analysis of genes related to patchoulol biosynthesis and regulation in Pogostemon cablin
    Chen, Xiuzhen
    Li, Junren
    Wang, Xiaobing
    Zhong, Liting
    Tang, Yun
    Zhou, Xuanxuan
    Liu, Yanting
    Zhan, Ruoting
    Zheng, Hai
    Chen, Weiwen
    Chen, Likai
    BMC PLANT BIOLOGY, 2019, 19 (1)
  • [8] Full-length transcriptome sequencing and methyl jasmonate-induced expression profile analysis of genes related to patchoulol biosynthesis and regulation in Pogostemon cablin
    Xiuzhen Chen
    Junren Li
    Xiaobing Wang
    Liting Zhong
    Yun Tang
    Xuanxuan Zhou
    Yanting Liu
    Ruoting Zhan
    Hai Zheng
    Weiwen Chen
    Likai Chen
    BMC Plant Biology, 19
  • [9] Transcriptome analysis of Artemisia argyi following methyl jasmonate (MeJA) treatment and the mining of genes related to the stress resistance pathway
    Wang, Jing
    Cui, Yupeng
    Li, Shuyan
    Gao, Xinqiang
    Zhang, Kunpeng
    Shen, Xiangling
    FRONTIERS IN GENETICS, 2023, 14
  • [10] Expression analysis of ginsenoside biosynthesis-related genes in methyl jasmonate-treated adventitious roots of Panax ginseng via DNA microarray analysis
    Um, Yurry
    Lee, Yi
    Kim, Seong-Cheol
    Jeong, Yeon-Ju
    Kim, Geum-Soog
    Choi, Dong-Woog
    Cha, Seon-Woo
    Kim, Ok-Tae
    HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, 2017, 58 (04) : 376 - 383