Molecular and physical characterization of grain hardness in European spring common wheat (Triticum aestivum L.)

Grain hardness is the single most important trait in determining technological properties and end-use quality of wheat product. This trait is controlled by two genes (Pina-D1 and Pinb-D1) at the Hardness (Ha) locus. Soft endosperm kernels are characterized by the presence of alleles 'a' in both genes (Pina-D1a and Pinb-D1a), while the medium hard and hard grain is the result of deletion in the Pina-D1 gene or single mutation of the Pinb-D1 gene. The aim of the current study was to determine the relationship between common wheat grain hardness and the presence of puroindoline genes. Eighty-one spring common wheat cultivars from Europe were analysed for grain hardness by SKCS (Single Kernel Characterization System) and Pin-D1 genes. The analysed genotypes were divided into three hardness classes: hard, medium and soft and they showed four allelic combinations in Pin-D1 genes. The SKCS results showed that hard wheat was the major type in European cultivars, whereas molecular analysis showed differential allelic combinations of puroindoline genes among these classes. The conducted analyses suggest that another major gene or other factors were influencing kernel texture.