Altered hippocampal microRNA expression profiles in neonatal rats caused by sevoflurane anesthesia: MicroRNA profiling and bioinformatics target analysis

被引：28

作者：

Ye, Jishi ^{[1
]}

Zhang, Zongze ^{[1
]}

Wang, Yanlin ^{[1
]}

Chen, Chang ^{[1
]}

Xu, Xing ^{[1
]}

Yu, Hui ^{[1
]}

Peng, Mian ^{[1
]}

机构：

[1] Wuhan Univ, Dept Anesthesiol, Zhongnan Hosp, 169 Donghu Rd, Wuhan 430071, Hubei, Peoples R China

来源：

EXPERIMENTAL AND THERAPEUTIC MEDICINE | 2016年 / 12卷 / 03期

基金：

中国国家自然科学基金;

关键词：

sevoflurane; microRNA; hippocampus; neonatal rat; bioinformatic analysis; ACTIVATED PROTEIN-KINASE; METHYL-D-ASPARTATE; VOLATILE ANESTHETICS; PROPOFOL ANESTHESIA; BEHAVIORAL DEFICITS; REPERFUSION INJURY; OXYGEN-SATURATION; MEMORY IMPAIRMENT; EARLY EXPOSURE; IN-VIVO;

D O I：

10.3892/etm.2016.3452

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Although accumulating evidence has suggested that microRNAs (miRNAs) have a serious impact on cognitive function and are associated with the etiology of several neuropsychiatric disorders, their expression in sevoflurane-induced neurotoxicity in the developing brain has not been characterized. In the present study, the miRNAs expression pattern in neonatal hippocampus samples (24 h after sevoflurane exposure) was investigated and 9 miRNAs were selected, which were associated with brain development and cognition in order to perform a bioinformatic analysis. Previous microfluidic chip assay had detected 29 upregulated and 24 downregulated miRNAs in the neonatal rat hippocampus, of which 7 selected deregulated miRNAs were identified by the quantitative polymerase chain reaction. A total of 85 targets of selected deregulated miRNAs were analyzed using bioinformatics and the main enriched metabolic pathways, mitogen-activated protein kinase and Wnt pathways may have been involved in molecular mechanisms with regard to neuronal cell body, dendrite and synapse. The observations of the present study provided a novel understanding regarding the regulatory mechanism of miRNAs underlying sevoflurane-induced neurotoxicity, therefore benefitting the improvement of the prevention and treatment strategies of volatile anesthetics related neurotoxicity.

引用

页码：1299 / 1310

页数：12

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