Hepatitis C virus-like particle budding:: Role of the core protein and importance of its Asp111

被引:50
作者
Blanchard, E
Hourioux, C
Brand, D
Ait-Goughoulte, M
Moreau, A
Trassard, S
Sizaret, PY
Dubois, F
Roingeard, P
机构
[1] Ctr Hosp Univ, Virol Lab, Tours, France
[2] Ctr Hosp Univ, Biol Cellulaire Lab, Tours, France
[3] Inst Inter Reg Sante, Fac Med, La Riche, France
关键词
D O I
10.1128/JVI.77.18.10131-10138.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In the absence of a hepatitis C virus (HCV) culture system, the use of a Semliki Forest virus replicon expressing genes encoding HCV structural proteins that assemble into HCV-like particles provides an opportunity to study HCV morphogenesis. Using this system, we showed that the HCV core protein constitutes the budding apparatus of the virus and that its targeting to the endoplasmic reticulum by means of the signal sequence of E1 protein is essential for budding. In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis.
引用
收藏
页码:10131 / 10138
页数:8
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