A polysaccharide from the stems of Rubus amabilis Focke and its immunological enhancement activity

被引:5
作者
Diai, Yu-Lin [1 ,2 ]
Shan, Jun-Jie [2 ]
Ma, Hao [2 ]
Zhang, Tong [3 ]
Liu, Bin [1 ]
机构
[1] Beijing Univ Chinese Med, Sch Tradit Chinese Med, Beijing 100102, Peoples R China
[2] Academy Mil Med Sci, Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
[3] Wuzhou Pharmaceut Grp Co Ltd, Wuzhou 543000, Peoples R China
关键词
Rubus amabilis; polysaccharide; immunological enhancement; ANTIOXIDANT ACTIVITY; PURIFICATION; ANTITUMOR; TEA;
D O I
10.1080/10286020.2016.1173675
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A water-soluble polysaccharide (named RAP) was newly isolated from the stems of Rubus amabilis. Structural confirmation of the polysaccharide was provided by hydrolysis, periodate oxidation, Smith degradation, and methylation analysis, combined with nuclear magnetic resonance (NMR), capillary electrophoresis (CE), infrared spectroscopy (IR), and gas chromatography-mass spectra (GC-MS). In vitro immunological enhancement activity was characterized using the proliferative activity of spleen lymphocytes and phagocytic activity of peritoneal macrophages in mice. The polysaccharide was mainly composed of xylose, arabinose, glucose, rhamnose, galactose, mannose, glucuronic acid, and galactocuronic acid in the molar ratio of 1.0:6.9:0.8:1.1:6.9:0.3:0.5:3.3, with the average molecular weight of 26.2 kDa. The linkage types of netural monosaccharides were as follows: the arabinose was -> 2) Ara (1 -> and galactose were Gal (1 ->,-> 3) Gal (1 ->,-> 3,6) Gal (1 ->,-> 2,3,6) Gal (1 -> and -> 2,3,6) Gal(f) (1 ->center dot Xyl (1 ->,-> 6) Glc (1 ->,-> 2) Glc (1 ->,-> 3) Rha (1 ->, Rha (1 -> and Man (1 -> were also found in the structure. RAP-B-2 could improve the proliferative activity of spleen T cells and B cells and boost phagocytic activity of peritoneal macrophages at the concentration of 50 mu g/ml (p < 0.05, p < 0.01).
引用
收藏
页码:897 / 907
页数:11
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