In vitro assessment of the toxicity of small silver nanoparticles and silver ions to the red blood cells

被引：19

作者：

Chi, Zhenxing ^{[1
,2
]}

Lin, Hongwei ^{[1
]}

Li, Weiguo ^{[1
]}

Zhang, Xunuo ^{[1
]}

Zhang, Qiang ^{[1
]}

机构：

[1] Harbin Inst Technol, Dept Environm Engn, 2 Wenhua West Rd, Weihai 264209, Peoples R China

[2] Jinan Univ, Sch Environm, Guangzhou Key Lab Environm Exposure & Hlth, Guangzhou 510632, Guangdong, Peoples R China

来源：

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | 2018年 / 25卷 / 32期

基金：

中国国家自然科学基金;

关键词：

Silver nanoparticles; Silver ions; Toxicity; Red blood cells; Hemoglobin; ATPase; BOVINE SERUM-ALBUMIN; PHTHALATE ACID-ESTERS; HUMAN HEPATOMA-CELLS; HUMAN-ERYTHROCYTES; ADSORPTION; BIOMOLECULES; PARTICLES; MECHANISM; HEMOLYSIS; PROTEINS;

D O I：

10.1007/s11356-018-3217-2

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This work reports the toxicity of small silver nanoparticles (nanoAg, 20nm) and silver ions (Ag+) to the red blood cells with the silver concentration level of 10(-6)g/mL. Results show that red blood cells (RBCs) start hemolysis when treated by nanoAg of 1.5x10(-5)g/mL or Ag+ of 2.9x10(-7)g/mL. A low ATPase activity of 30% has been observed after RBCs being treated with Ag+ of 2.6x10(-7)g/mL, while the nanoAg does not obviously affect the ATPase activity. In molecular level, Ag+ is more toxic to the amino acid residues than nanoAg according to the change of fluorescence characteristics of hemoglobin (Hb). However, the nanoAg has been found to be more toxic than Ag+ to the secondary structure of Hb in terms of the loss of -helix content.

引用

页码：32373 / 32380

页数：8

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