Amplified Aptamer-Based Assay through Catalytic Recycling of the Analyte

被引：228

作者：

Lu, Chun-Hua ^{[1
]}

Li, Juan ^{[1
]}

Lin, Mei-Hua ^{[1
]}

Wang, Yi-Wei ^{[1
]}

Yang, Huang-Hao ^{[1
]}

Chen, Xi ^{[1
]}

Chen, Guo-Nan ^{[1
]}

机构：

[1] Fuzhou Univ, Key Lab Anal & Detect Technol Food Safety, MOE, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Fuzhou 350002, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 45期

基金：

中国国家自然科学基金;

关键词：

aptamers; biological assays; graphene; nanomaterials; nucleases; GENE-REGULATION; NANOPARTICLES; GRAPHENE; PROBES; DELIVERY; COCAINE; SENSORS; BIOLOGY; PROTEIN; CELLS;

D O I：

10.1002/anie.201002822

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Turn up the volume: In a simple and highly sensitive aptamer-based assay that relies on the ability of nanomaterials to protect DNA from nuclease cleavage, the aptamer is released from the nanomaterial when challenged with a target. The nuclease then cleaves the free aptamer and releases the target, which can initiate a new cycle. Recycling of the target in this way leads to significant signal amplification (see diagram). © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：8454 / 8457

页数：4

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