The Diagnostic Potential of Monoclonal Antibodies to Adenovirus

被引：1

作者：

Timoshicheva, T. A. ^{[1
]}

Amosova, I. V. ^{[1
]}

Grudinin, M. P. ^{[1
]}

机构：

[1] Minist Hlth Russian Federat, Smorodintsev Res Inst Influenza, St Petersburg 197376, Russia

来源：

APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2022年 / 58卷 / 07期

关键词：

monoclonal antibodies; human adenovirus; adenovirus infection; diagnosis; RESPIRATORY VIRAL-INFECTIONS; VIRUSES; ASSAY;

D O I：

10.1134/S0003683822070079

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The diagnostic potential of the 4B7 and 6B12 monoclonal antibodies (MAbs) against human adenovirus hexon protein has been studied in various immunological tests, namely, the in-cell enzyme-linked immunosorbent assay (cell-ELISA), sandwich ELISA, and the immunofluorescence antibody technique (IFA). It was shown that the sensitivities of cell-ELISA, sandwich ELISA, and IFA were 96, 86, and 84%, respectively, compared to PCR. Thus, 4B7 and 6B12 MAbs are promising immunoreagents for the construction of various diagnostic kits to use in laboratory practice for adenovirus detection in clinical samples.

引用

页码：873 / 877

页数：5

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