A simple and efficient high-performance liquid chromatographic assay for etomidate in plasma

被引：17

作者：

McIntosh, MP ^{[1
]}

Rajewski, RA ^{[1
]}

机构：

[1] Univ Kansas, Ctr Drug Delivery Res, Lawrence, KS 66047 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2001年 / 24卷 / 04期

关键词：

etomidate; plasma; high-performance liquid chromatography;

D O I：

10.1016/S0731-7085(00)00471-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development and validation of an effective and simplified LC assay for the quantitation of etomidate in beagle plasma is described. The methodology employs a rapid and simple protein precipitation procedure in combination with previously reported chromatographic conditions. Using a 0.3 mi aliquot of plasma, the assay is linear in the concentration range of 50 to 5000 ng/ml, with an extraction efficiency between 97 to 104% and accuracy between 98 and 105%. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：689 / 694

页数：6

共 12 条

[1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR ETOMIDATE IN HUMAN-PLASMA - RESULTS OF PRELIMINARY CLINICAL-STUDIES USING ETOMIDATE FOR HYPNOSIS IN TOTAL INTRAVENOUS ANESTHESIA [J].

AVRAM, MJ ;

FRAGEN, RJ ;

LINDE, HW .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1983, 72 (12) :1424-1426

[2] Relationship between etomidate plasma concentration and EEG effect in the rat [J].

De Paepe, P ;

Van Hoey, G ;

Belpaire, FM ;

Rosseel, MT ;

Boon, PA ;

Buylaert, WA .

PHARMACEUTICAL RESEARCH, 1999, 16 (06) :924-929

[3] ASSAY OF ETOMIDATE IN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH NITROGEN-SELECTIVE DETECTION [J].

DEBOER, AG ;

SMEEKENS, JB ;

BREIMER, DD .

JOURNAL OF CHROMATOGRAPHY, 1979, 162 (04) :591-595

[4]

DOENICKE A, 1994, ANESTH ANALG, V79, P933

[5] SYMPATHETIC RESPONSES TO INDUCTION OF ANESTHESIA IN HUMANS WITH PROPOFOL OR ETOMIDATE [J].

EBERT, TJ ;

MUZI, M ;

BERENS, R ;

GOFF, D ;

KAMPINE, JP .

ANESTHESIOLOGY, 1992, 76 (05) :725-733

[6] DETERMINATION OF ETOMIDATE IN HUMAN-PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

ELLIS, EO ;

BECK, PR .

JOURNAL OF CHROMATOGRAPHY, 1982, 232 (01) :207-211

[7]

HARING CMM, 1979, ACTA ANAESTH BELG, V31, P107

[8] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ETOMIDATE IN PLASMA [J].

LEMOING, JP ;

LEVRON, JC .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 529 (01) :217-222

[9] MASS FRAGMENTOGRAPHIC DETERMINATION OF PLASMA ETOMIDATE CONCENTRATIONS [J].

VANHAMME, MJ ;

AMBRE, JJ ;

GHONEIM, MM .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1977, 66 (09) :1344-1346

[10] PHARMACOKINETICS OF ETOMIDATE, A NEW INTRAVENOUS ANESTHETIC [J].

VANHAMME, MJ ;

GHONEIM, MM ;

AMBRE, JJ .

ANESTHESIOLOGY, 1978, 49 (04) :274-277

← 1 2 →