Third generation horseradish peroxidase biosensor based on self-assembling carbon nanotubes to gold electrode surface

被引：0

作者：

Xu, JJ ^{[1
]}

Wang, G ^{[1
]}

Zhang, Q ^{[1
]}

Xia, XH ^{[1
]}

Chen, HY ^{[1
]}

机构：

[1] Nanjing Univ, Dept Chem, Lab Life Analyt Chem, Nanjing 210093, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2005年 / 16卷 / 04期

关键词：

horseradish peroxidase; MWNT; self-assembly; third-generation biosensor;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A third-generation horseradish peroxidase (HRP) biosensor has been developed by adsorbing HRP on multi-wall carbon nanotube (MWNTs) monolayer modified gold electrode surface. The assembly process was investigated by electrochemical and spectroscopic techniques. Results showed that the immobilized HRP exhibited direct electrochemical behavior toward the reduction of H2O2. The resulting biosensor shows a fast amperometric response (< 2 s) to H2O2. The linear response range was from 50x10(-7)similar to 1.0x10(-5) mol/L with a detection limit of 1.0x10(-7) mol/L. Moreover, the biosensor has a good reproducibility, and long-term stability.

引用

页码：523 / 526

页数：4

共 13 条

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