Effect of small intestinal submucosa sponges on the attachment and proliferation behavior of Schwann cells

被引:7
作者
Han, Kap-Soo [1 ]
Ko, Hyun Ah [2 ,3 ]
Jang, Na Keum [2 ,3 ]
Song, Jeong Eun [2 ,3 ]
Khang, Gilson [2 ,3 ]
机构
[1] Chonbuk Natl Univ, Div Biomed Engn, Jeonju 561756, South Korea
[2] Chonbuk Natl Univ, Dept BIN Fus Tech, Polymer Fus Res Ctr, Jeonju 561756, South Korea
[3] Chonbuk Natl Univ, Dept Polymer Nano Sci Tech, Jeonju 561756, South Korea
关键词
Schwann cell; small intestinal submucosa; scaffold; nerve regeneration; NERVE REGENERATION; DIFFERENTIATION; SCAFFOLDS; GRAFTS; GROWTH; REPAIR; BONE;
D O I
10.1007/s13233-014-2175-x
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
The aim of the study was to investigate the effects of small intestinal submucosa (SIS) sponges, a natural biodegradable polymer scaffold, on the adhesion behavior and the proliferation of Schwann cells (SCs). SIS sponges were prepared by varying the contents of SIS powders (1%, 2%, and 3% SIS concentration) and SCs were seeded on the sponges and incubated to investigate the adhesion and proliferation of SCs. A series of analytical process was conducted to observe the water absorption rate of sponges and confirmed the proliferation and morphology of SCs by scanning electron microscopy (SEM), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay, reverse transcription polymerase chain reaction (RT-PCR), and histological evaluation. The best adhesion behavior and the proliferation of SCs were observed on the sponge with 2% powder content. Also, analysis of messenger ribonucleic acid (mRNA) expression using the neuron-specific enolase (NSE) and neurofilament protein (NF) nerve markers showed that SC gene expression was excellent in the 2% SIS sponge. In vivo hematoxylin and eosin (H&E) staining and immunofluorescence results showed that cells and tissue formed well in the pores of sponges with 2% and 3% SIS powder contents, compared with formation in the 1% SIS sponge. The SIS sponges provided an unsuitable habitat for the adhesion and proliferation of cells in that the pore size was small. In this study, the adhesion behavior and the proliferation could be changed depending on the pore size and SIS powder content in the sponges. Thus, an appropriate powder content of SIS should be used to prepare SIS sponge scaffolds.
引用
收藏
页码:1253 / 1260
页数:8
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