Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A

被引：47

作者：

Chen, Zhenhai ^{[1
]}

Yuan, Fangfeng ^{[1
,2
]}

Li, Yanhua ^{[1
]}

Shang, Pengcheng ^{[1
]}

Schroeder, Robin ^{[3
]}

Lechtenberg, Kelly ^{[3
]}

Henningson, Jamie ^{[1
,2
]}

Hause, Benjamin ^{[1
,2
]}

Bai, Jianfa ^{[1
,2
]}

Rowland, Raymond R. R. ^{[1
]}

Clavijo, Alfonso ^{[1
,2
]}

Fang, Ying ^{[1
,2
]}

机构：

[1] Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA

[2] Kansas State Univ, Coll Vet Med, Kansas State Vet Diagnost Lab, Manhattan, KS 66506 USA

[3] Midwest Vet Serv Inc, Oakland, NE 68045 USA

来源：

VIROLOGY | 2016年 / 497卷

关键词：

Senecavirus A; Picornavirus infection; Infectious clone; Reverse genetics; EGFP reporter virus; Swine; Vesicular lesion; Vesicular disease; SVA pathogenesis; RESPIRATORY SYNDROME VIRUS; IDIOPATHIC VESICULAR DISEASE; ONCOLYTIC PICORNAVIRUS; VALLEY-VIRUS; NONSTRUCTURAL PROTEINS; MOLECULAR DETERMINANTS; COXSACKIEVIRUS B1; COMPLETE GENOME; VIRULENCE; REGION;

D O I：

10.1016/j.virol.2016.07.003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A full-length cDNA infectious clone, pKS15-01-Clone, was constructed from an emerging Senecavirus A (SVA; strain KS15-01). To explore the potential use as a viral backbone for expressing marker genes, the enhanced green fluorescent protein (EGFP)-tagged reporter virus (vKS15-01-EGFP) was generated using reverse genetics. Compared to the parental virus, the pKS15-01-Clone derived virus (vKS15-01-Clone) replicated efficiently in vitro and in vivo, and induced similar levels of neutralizing antibody and cytokine responses in infected animals. In contrast, the vKS15-01-EGFP virus showed impaired growth ability and induced lower level of immune response in infected animals. Lesions on the dorsal snout and coronary bands were observed in all pigs infected by parental virus KS15-01, but not in pigs infected with vKS15-01-Clone or vKS15-01-EGFP viruses. These results demonstrated that the infectious clone and EGFP reporter virus could be used as important tools in further elucidating the SVA pathogenesis and development of control measures. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：111 / 124

页数：14

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