Complete genome sequence of an isolate of Potato virus X (PVX) infecting Cape gooseberry (Physalis peruviana) in Colombia

被引:15
|
作者
Gutierrez, Pablo A. [1 ]
Alzate, Juan F. [2 ]
Marin Montoya, Mauricio [3 ]
机构
[1] Univ Nacl Colombia Sede Medellin, Fac Ciencias, Lab Microbiol Ind, Medellin, Colombia
[2] Univ Antioquia, Fac Med, CNSG, Grp Parasitol, Medellin, Colombia
[3] Univ Nacl Colombia Sede Medellin, Fac Ciencias, Lab Biol Celular & Mol, Medellin, Colombia
关键词
Genomics; Next generation sequencing; Physalis peruviana; Potexvirus; PROTEIN; REGION; GENE; MOVEMENT; CELLS;
D O I
10.1007/s11262-015-1181-1
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Transcriptome analysis of a Cape gooseberry (Physalis peruviana) plant with leaf symptoms of a mild yellow mosaic typical of a viral disease revealed an infection with Potato virus X (PVX). The genome sequence of the PVX-Physalis isolate comprises 6435 nt and exhibits higher sequence similarity to members of the Eurasian group of PVX (similar to 95 %) than to the American group (similar to 77 %). Genome organization is similar to other PVX isolates with five open reading frames coding for proteins RdRp, TGBp1, TGBp2, TGBp3, and CP. 5' and 3' untranslated regions revealed all regulatory motifs typically found in PVX isolates. The PVX-Physalis genome is the only complete sequence available for a Potexvirus in Colombia and is a new addition to the restricted number of available sequences of PVX isolates infecting plant species different to potato.
引用
收藏
页码:518 / 522
页数:5
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