Adipose-Derived Stem Cells Preincubated with Green Tea EGCG Enhance Pancreatic Tissue Regeneration in Rats with Type 1 Diabetes through ROS/Sirt1 Signaling Regulation

被引:12
作者
Chen, Tung-Sheng [1 ]
Liao, Wei-Yu [2 ,3 ]
Huang, Chi-Wen [2 ,4 ]
Chang, Chin-Hsien [2 ,3 ,5 ]
机构
[1] Natl Taiwan Normal Univ, Grad Program Biotechnol & Pharmaceut Ind, Taipei 106, Taiwan
[2] En Chu Kong Hosp, Tradit Chinese Med Dept, New Taipei 237, Taiwan
[3] Chang Gung Univ Sci & Technol, Dept Cosmet Sci, Taoyuan 333, Taiwan
[4] Taipei Med Univ, Coll Pharm, PhD Program Clin Drug Dev Herbal Med, Taipei 110, Taiwan
[5] China Med Univ, Coll Chinese Med, Taichung 404, Taiwan
关键词
green tea EGCG; mesenchymal stem cells; pancreatic regeneration; survival p-Akt; EPIGALLOCATECHIN-3-GALLATE EGCG; PATHWAY ACTIVATION; OXIDATIVE STRESS; EXPRESSION; DIFFERENTIATION; APOPTOSIS; DISEASE;
D O I
10.3390/ijms23063165
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Type 1 diabetes stem-cell-based therapy is one of the best therapeutic approaches for pancreatic damage treatment due to stem cell tissue regeneration. Epigallocatechin gallate (EGCG) is one of the active components found in green tea. Experimental results suggest that EGCG shows beneficial effects on cell protection. This study explores whether a better pancreatic regeneration therapeutic effect could be found in mesenchymal stem cells pretreated with EGCG compared to stem cells without EGCG pretreatment. A cell model confirmed that adipose-derived stem cells (ADSC) incubated with EGCG increase cell viability under high-glucose (HG) stress. This is due to survival marker p-Akt expression. In an animal model, type 1 diabetes induced the activation of several pathological signals, including islet size reduction, extracellular fibrotic collagen deposition, oxidative stress elevation, survival pathway suppression, apoptosis signaling induction, and Sirt1 antioxidant pathway downregulation. Ordinary ADSC transplantation slightly improved the above pathological signals. Further, EGCG-pretreated ADSC transplantation significantly improved the above pathological conditions. Taken together, EGCG-pretreated ADSCs show clinical potential in the treatment of patients with type 1 diabetes through the regeneration of damaged pancreatic tissues.
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页数:17
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