Vinclozolin modulates hepatic cytochrome P450 isoforms during pregnancy

被引：4
作者：
Garcia-Montes de Oca, Felix Genoveva [1 ]
de Lourdes Lopez-Gonzalez, Ma. [1 ]
Constanza Escobar-Wilches, Deny [1 ]
Chavira-Ramirez, Roberto [2 ]
Sierra-Santoyo, Adolfo [1 ]
机构：
[1] IPN, CINVESTAV, Ctr Invest & Estudios Avanzados, Dept Toxicol, Mexico City 07360, DF, Mexico
[2] Inst Nacl Ciencias Med & Nutr Salvador Zubiran, Mexico City 14000, DF, Mexico
来源：
REPRODUCTIVE TOXICOLOGY | 2015年 / 53卷
关键词：
Vinclozolin; Cytochrome P450; Endocrine disruption; Pregnancy; Testosterone; Estradiol; ENDOCRINE-DISRUPTING CHEMICALS; MALE-RAT; FUNGICIDE VINCLOZOLIN; TRANSCRIPTIONAL REGULATION; NUCLEAR RECEPTORS; X-RECEPTOR; EXPRESSION; MOUSE; LIVER; INDUCTION;
D O I：
10.1016/j.reprotox.2015.04.010
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Vinclozolin (V) is classified as a potent endocrine disruptor. The aim of the present study was to determine the effects of Von rat liver CYP regulation and on serum levels of testosterone and estradiol during pregnancy. Pregnancy decreased the liver total CYP content by 65%, enzyme activities of MROD, PROD, and PNPH, and testosterone hydroxylation activities, as well as the protein content of CYP2A and 3A. V exposure remarkably induced the protein content and enzyme activities of CYP1A, 2A, 2B and 3A subfamilies. Testosterone and estradiol were affected in an opposite manner, provoking a 3.5-fold increase in the estradiol/testosterone ratio. These results suggest that V could regulate the hepatic CYP expression through interaction with receptors and coactivators involved in its expression and may play an important role in hormonal balance during pregnancy. In addition, the results may also contribute to understanding the toxicity of V by in utero exposure. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：119 / 126
页数：8
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