The role of osteomodulin on osteo/odontogenic differentiation in human dental pulp stem cells

被引：23
作者：
Lin, Wenzhen [1 ,2 ,3 ,4 ]
Gao, Li [1 ,2 ,3 ,4 ]
Jiang, Wenxin [1 ,2 ,3 ,4 ]
Niu, Chenguang [1 ,2 ,3 ,4 ]
Yuan, Keyong [1 ,2 ,3 ,4 ]
Hu, Xuchen [1 ,2 ,3 ,4 ]
Ma, Rui [1 ,2 ,3 ,4 ]
Huang, Zhengwei [1 ,2 ,3 ,4 ]
机构：
[1] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Dept Endodont, Coll Stomatol,Sch Med, Shanghai, Peoples R China
[2] Natl Clin Res Ctr Oral Dis, Shanghai, Peoples R China
[3] Shanghai Key Lab Stomatol, Shanghai, Peoples R China
[4] Shanghai Res Inst Stomatol, Shanghai, Peoples R China
来源：
BMC ORAL HEALTH | 2019年 / 19卷 / 1期
基金：
中国国家自然科学基金;
关键词：
Human dental pulp stem cells; Osteomodulin; Osteo; odontoblastic differentiation; KERATAN SULFATE PROTEOGLYCAN; ODONTOBLASTIC DIFFERENTIATION; OSTEOGENIC DIFFERENTIATION; BONE-MARROW; OSTEOADHERIN; EXPRESSION; PROTEINS; SIALOPHOSPHOPROTEIN; IDENTIFICATION; PROLIFERATION;
D O I：
10.1186/s12903-018-0680-6
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
BackgroundExtracellular matrix secretion and odontoblastic differentiation in human dental pulp stem cells (hDPSCs) are the cellular bases for reparative dentinogenesis. Osteomodulin (OMD) is a member of the small leucine-rich proteoglycan family distributed in the extracellular matrix but little is known about its role in osteo/odontogenic differentiation. The objective of this study was to investigate the role of OMD during osteo/odontoblastic differentiation of hDPSCs.MethodshDPSCs were selected using immune-magnetic beads and their capability of multi-differentiation was identified. OMD knockdown was achieved using short hairpin RNA (shRNA) lentivirus and was confirmed by western blot. Gene expression was measured by real-time qPCR and osteo/odontoblastic differentiation of hDPSCs was determined by alizarin red S staining.ResultsCompared with uninduced cells, the transcription of OMD was up-regulated by 35-fold at the late stage of osteo/odontogenic differentiation. shRNA-mediated gene silencing of OMD decreased the expression of odontoblastic genes, such as alkaline phosphatase (ALP), dentin matrix acidic phosphoprotein 1 (DMP1) and dentin sialophosphoprotein (DSPP). Besides, knockdown of OMD attenuated the mineralized nodules formation induced by osteo/odontogenic medium.ConclusionsThese results implied that OMD may play a pivotal role in modulating the osteo/odontoblastic differentiation of hDPSCs.
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页数：8
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