Anti-Inflammatory Activity of Bryophytes Extracts in LPS-Stimulated RAW264.7 Murine Macrophages

被引:16
作者
Marques, Raissa Volpatto [1 ]
Sestito, Stefania Enza [2 ]
Bourgaud, Frederic [3 ,4 ]
Miguel, Sissi [4 ]
Cailotto, Frederic [2 ]
Reboul, Pascal [2 ]
Jouzeau, Jean-Yves [2 ]
Rahuel-Clermont, Sophie [2 ]
Boschi-Muller, Sandrine [2 ]
Simonsen, Henrik Toft [1 ]
Moulin, David [2 ]
机构
[1] Tech Univ Denmark, Dept Biotechnol & Biomed, Soltoft Plads 223, DK-2800 Lyngby, Denmark
[2] Univ Lorraine, Ingn Mol & Physiopathol Articulaire IMoPA, CNRS, UMR 7365, F-54500 Vandoeuvre Les Nancy, France
[3] Plant Adv Technol, 19 Ave Foret Haye, F-54500 Vandoeuvre Les Nancy, France
[4] Cellengo, 19 Ave Foret Haye, F-54500 Vandoeuvre Les Nancy, France
基金
欧盟地平线“2020”;
关键词
bryophytes; mosses; Dicranum majus; Thuidium delicatulum; anti-inflammatory activity; nitric oxide; NF-KAPPA-B; INFLAMMATION; PEROXIREDOXINS; IMMUNITY; ROLES;
D O I
10.3390/molecules27061940
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bryophytes produce rare and bioactive compounds with a broad range of therapeutic potential, and many species are reported in ethnomedicinal uses. However, only a few studies have investigated their potential as natural anti-inflammatory drug candidate compounds. The present study investigates the anti-inflammatory effects of thirty-two species of bryophytes, including mosses and liverworts, on Raw 264.7 murine macrophages stimulated with lipopolysaccharide (LPS) or recombinant human peroxiredoxin (hPrx1). The 70% ethanol extracts of bryophytes were screened for their potential to reduce the production of nitric oxide (NO), an important pro-inflammatory mediator. Among the analyzed extracts, two moss species significantly inhibited LPS-induced NO production without cytotoxic effects. The bioactive extracts of Dicranum majus and Thuidium delicatulum inhibited NO production in a concentration-dependent manner with IC50 values of 1.04 and 1.54 mu g/mL, respectively. The crude 70% ethanol and ethyl acetate extracts were then partitioned with different solvents in increasing order of polarity (n-hexane, diethyl ether, chloroform, ethyl acetate, and n-butanol). The fractions were screened for their inhibitory effects on NO production stimulated with LPS at 1 ng/mL or 10 ng/mL. The NO production levels were significantly affected by the fractions of decreasing polarity such as n-hexane and diethyl ether ones. Therefore, the potential of these extracts to inhibit the LPS-induced NO pathway suggests their effective properties in attenuating inflammation and could represent a perspective for the development of innovative therapeutic agents.
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页数:14
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