Integrating cell sheets for organ-on-a-chip applications

被引：0
作者：
Loewenhardt, William [1 ]
Saunders, Rachel [2 ]
Lennon, Rachel [3 ]
Derby, Brian [1 ,2 ]
机构：
[1] Univ Manchester, Sch Mat, Manchester M13 9PL, Lancs, England
[2] Univ Manchester, Manchester Ctr Digital Fabricat, Manchester M13 9PL, Lancs, England
[3] Univ Manchester, Sch Biol Sci, Manchester M13 9PL, Lancs, England
来源：
3RD CIRP CONFERENCE ON BIOMANUFACTURING | 2017年 / 65卷
基金：
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词：
cell sheet engineering; PIPAAm; temperature responsive; PDMS; bioreactor; kidney; microfluidics; organ on a chip; ELECTRODES; RESISTANCE; BARRIERS;
D O I：
10.1016/j.procir.2017.04.018
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor. (C) 2016 Published by Elsevier B.V.
引用
收藏
页码：127 / 130
页数：4
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