Characterization of a Novel IMP-28 Metallo-β-Lactamase from a Spanish Klebsiella oxytoca Clinical Isolate

被引：11

作者：

Jose Perez-Llarena, Francisco ^{[1
]}

Fernandez, Ana ^{[1
]}

Zamorano, Laura ^{[2
]}

Kerff, Frederic ^{[3
]}

Beceiro, Alejandro ^{[1
]}

Aracil, Belen ^{[4
]}

Cercenado, Emilia ^{[5
]}

Miro, Elisenda ^{[6
]}

Oliver, Antonio ^{[2
]}

Oteo, Jesus ^{[4
]}

Navarro, Ferran ^{[6
]}

Bou, German ^{[1
]}

机构：

[1] Complejo Hosp Univ A Coruna, Serv Microbiol INIBIC, La Coruna, Spain

[2] Hosp Univ Son Espases, Microbiol Serv, Palma De Mallorca, Spain

[3] Univ Liege, Ctr Ingn Prot, Liege, Belgium

[4] Ctr Nacl Microbiol Virol & Inmunol Sanitarias Maja, Lab Antibiat, Madrid, Spain

[5] Hosp Gen Univ Gregorio Maranon, Microbiol Serv, Madrid, Spain

[6] Univ Autonoma Barcelona, Microbiol Serv, Hosp Santa Creu i St Pau, E-08193 Barcelona, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2012年 / 56卷 / 08期

关键词：

BIOCHEMICAL-CHARACTERIZATION; INTEGRON; GENE; RESISTANCE; CARBAPENEMS; BLA(IMP-1); VARIANT; LOOP;

D O I：

10.1128/AAC.00776-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An isolate of Kkbsiella oxytoca carrying a novel IMP metallo-beta-lactamase was discovered in Madrid, Spain. The bla(IMP-28) gene is part of a chromosomally located class I integron. The IMP-28 k(cat)/K-m values for ampicillin, ceftazidime, and cefepime and, to a lesser extent, imipenem and meropenem, are clearly lower than those of IMP-1. The His306Gln mutation may induce important modifications of the L3 loop and thus of substrate accessibility and hydrolysis and be the main reason for this behavior.

引用

页码：4540 / 4543

页数：4

共 18 条

[1] Genetic Context and Biochemical Characterization of the IMP-18 Metallo-β-Lactamase Identified in a Pseudomonas aeruginosa Isolate from the United States [J].

Borgianni, Luisa ;

Prandi, Silvia ;

Salden, Laurie ;

Santella, Gisela ;

Hanson, Nancy D. ;

Rossolini, Gian Maria ;

Docquier, Jean-Denis .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (01) :140-145

[2]

Bush K, 2001, CLIN INFECT DIS, V32, P1085, DOI 10.1086/319610

[3] IMP-12, a new plasmid-encoded metallo-β-lactamase from a Pseudomonas putida clinical isolate [J].

Docquier, JD ;

Riccio, ML ;

Mugnaioli, C ;

Luzzaro, F ;

Endimiani, A ;

Toniolo, A ;

Amicosante, G ;

Rossolini, GM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (05) :1522-1528

[4] Acquisition and diffusion of blaCTX-M-9 gene by R478-IncHI2 derivative plasmids [J].

Garcia, Aurora ;

Navarro, Ferran ;

Miro, Elisenda ;

Villa, Laura ;

Mirelis, Beatriz ;

Coll, Pere ;

Carattoli, Alessandra .

FEMS MICROBIOLOGY LETTERS, 2007, 271 (01) :71-77

[5] Metallo-β-lactamase gene blaIMP-15 in a class 1 integron, In95, from Pseudomonas aeruginosa clinical isolates from a hospital in Mexico [J].

Garza-Ramos, U. ;

Morfin-Otero, R. ;

Sader, H. S. ;

Jones, R. N. ;

Hernandez, E. ;

Rodriguez-Noriega, E. ;

Sanchez, A. ;

Carrillo, B. ;

Esparza-Ahumada, S. ;

Silva-Sanchez, J. .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2008, 52 (08) :2943-2946

[6] Amino acid substitutions in a variant of IMP-1 metallo-β-lactamase [J].

Iyobe, S ;

Kusadokoro, H ;

Ozaki, J ;

Matsumura, N ;

Minami, S ;

Haruta, S ;

Sawai, T ;

O'Hara, K .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (08) :2023-2027

[7] Biochemical characterization of the Pseudomonas aeruginosa 101/1477 metallo-β-lactamase IMP-1 produced by Escherichia coli [J].

Laraki, N ;

Franceschini, N ;

Rossolini, GM ;

Santucci, P ;

Meunier, C ;

de Pauw, E ;

Amicosante, G ;

Frère, JM ;

Galleni, M .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (04) :902-906

[8] Structure of In31, a blaIMP-containing Pseudomonas aeruginosa integron phyletically related to In5, which carries an unusual array of gene cassettes [J].

Laraki, N ;

Galleni, M ;

Thamm, I ;

Riccio, ML ;

Amicosante, G ;

Frère, JM ;

Rossolini, GM .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (04) :890-901

[9] GENOMIC MAPPING WITH I-CEU I, AN INTRON-ENCODED ENDONUCLEASE SPECIFIC FOR GENES FOR RIBOSOMAL-RNA, IN SALMONELLA SPP, ESCHERICHIA-COLI, AND OTHER BACTERIA [J].

LIU, SL ;

HESSEL, A ;

SANDERSON, KE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) :6874-6878

[10] A tripeptide deletion in the R2 loop of the class C β-lactamase enzyme FOX-4 impairs cefoxitin hydrolysis and slightly increases susceptibility to β-lactamase inhibitors [J].

Mallo, Susana ;

Perez-Llarena, Francisco J. ;

Kerff, Frederic ;

Soares, Nelson C. ;

Galleni, Moreno ;

Bou, German .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2010, 65 (06) :1187-1194

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