Leishmanicidal and antitumoral activities of endophytic fungi associated with the Antarctic angiosperms Deschampsia antarctica Desv. and Colobanthus quitensis (Kunth) Bartl.

被引：56

作者：

Santiago, Iara F. ^{[1
]}

Alves, Tania M. A. ^{[2
]}

Rabello, Ana ^{[3
]}

Sales Junior, Policarpo A. ^{[4
]}

Romanha, Alvaro J. ^{[4
]}

Zani, Carlos L. ^{[2
]}

Rosa, Carlos A. ^{[1
]}

Rosa, Luiz H. ^{[1
]}

机构：

[1] Univ Fed Minas Gerais, Dept Microbiol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Fundaco Oswaldo Cruz, Lab Quim Prod Nat, Belo Horizonte, MG, Brazil

[3] Fundaco Oswaldo Cruz, Lab Pesquisas Clin, Belo Horizonte, MG, Brazil

[4] Fundaco Oswaldo Cruz, Lab Parasitol & Celular Mol, Ctr Pesquisas Rene Rachou, Belo Horizonte, MG, Brazil

来源：

EXTREMOPHILES | 2012年 / 16卷 / 01期

关键词：

Antarctica; Bioprospecting; Leishmania; Secondary metabolites; Antitumoral activities; DIVERSITY; SEARCH; SOILS;

D O I：

10.1007/s00792-011-0409-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A total of 564 isolates of endophytic fungi were recovered from the plants Deschampsia antarctica and Colobanthus quitensis collected from Antarctica. The isolates were screened against parasites Leishmania amazonensis and Trypanosoma cruzi and against the human tumour cell lines. Of the 313 fungal isolates obtained from D. antarctica and 251 from C. quitensis, 25 displayed biological activity. Nineteen extracts displayed leishmanicidal activity, and six inhibited the growth of at least one tumour cell line. These fungi belong to 19 taxa of the genera Alternaria, Antarctomyces, Cadophora, Davidiella, Helgardia, Herpotrichia, Microdochium, Oculimacula, Phaeosphaeria and one unidentified fungus. Extracts of 12 fungal isolates inhibited the proliferation of L. amazonesis at a low IC50 of between 0.2 and 12.5 mu g ml(-1). The fungus Phaeosphaeria herpotrichoides displayed only leishmanicidal activity with an IC50 of 0.2 mu g ml(-1), which is equivalent to the inhibitory value of amphotericin B. The extract of Microdochium phragmitis displayed specific cytotoxic activity against the UACC-62 cell line with an IC50 value of 12.5 mu g ml(-1). Our results indicate that the unique angiosperms living in Antarctica shelter an interesting bioactive fungal community that is able to produce antiprotozoal and antitumoral molecules. These molecules may be used to develop new leishmanicidal and anticancer drugs.

引用

页码：95 / 103

页数：9

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