High throughput isolation of DNA and RNA in 96-well format using a paint shaker

被引：12

作者：

Michaels, SD ^{[1
]}

Amasino, RM ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 2001年 / 19卷 / 03期

基金：

美国国家科学基金会;

关键词：

96-well format; DNA isolation; high throughput; paint shaker; RNA isolation;

D O I：

10.1007/BF02772894

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Most steps in plant nucleic acid isolation are easily adapted to 96-well format; however, tissue disruption typically is performed on samples individually and often is the rate-limiting step in sample processing. We have found that DNA and RNA isolation from Arabidopsis tissue can be carried out in 96-well format using a paint shaker fitted with an adapter for tissue disruption.

引用

页码：227 / 233

页数：7